Development of two new molecular markers specific to cytoplasmic male sterility in tuber mustard (Brassica juncea var. tumida Tsen et Lee)
Development of two new molecular markers specific to cytoplasmic male sterility in tuber mustard (Brassica juncea var. tumida Tsen et Lee)
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开发两种芥菜细胞质雄性不育特异性新分子标记（Brassica juncea var. tumida Tsen et Lee）
DOI：
10.1007/s10681-008-9817-z
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发表时间：
2009-04
期刊：
影响因子：
1.9
通讯作者：
中科院分区：
文献类型：
--
作者：
通过10年的回交，培育出一种新的、稳定的块茎芥细胞质雄性不育不育系。我们在之前的研究中克隆了两个特异性标记atpA和orf220，并对其进行了部分鉴定（Zhang et al. 2003）。本研究分离鉴定了两个新的分子标记orf256和orf305/orf324。在CMS系中发现orf256基因大小为825 bp，在保持系中发现orf256基因大小为1357 bp。序列分析表明orf256基因是一个完整的编码序列，位于cox1基因的下游。有趣的是，906 bp的片段，包含orf222、nad5和orf139基因的部分序列，被发现是从1357 bp片段的5 ' -翼的451 bp插入的。用同样的方法从CMS系分离到orf324基因，从其保持系分离到orf305基因。它们都是完整的编码序列，位于nad3和rps12基因的上游，并与nad3和rps12基因共转录。此外，orf256和orf324/orf305两个分子标记已成功转化为SCAR标记。随后，预测ORF256、ORF324、ORF305蛋白和ORF256- m -431片段含有信号肽序列，预测ORF220含有信号锚定序列。RFLP分析结果显示，所有分子标记均存在多态性。Northern blot分析表明，这些基因在CMS系中的表达量高于保持系。在质量上，所有这些基因在叶片中的表达量都低于CMS系与其保持系之间花器官的表达量。本研究结果也为探索这些特异标记基因在芥菜中的生物学功能提供了一些新的有用线索。
A novel and stable cytoplasmic male sterility CMS line of tuber mustard has been bred by subsequent backcrosses for 10 years. Two specific markers atpA and orf220 were cloned and partially characterized in our previous study (Zhang et al. 2003). In this study, two new molecular markers, orf256 and orf305/orf324, have been isolated and identified. The orf256 gene size was found to be 825 bp in CMS line and a 1,357 bp in its maintainer line. Sequence analysis indicated that the orf256 gene was an entire coding sequence and downstream of the cox1 gene. Interestingly, the 906 bp fragment, which contains part of the sequence of orf222, nad5 and orf139 genes, was found to be inserted from the 451st bp of 5′-flank of the 1,357 bp fragment. In the same way, the orf324 gene was isolated from CMS line and orf305 gene from its maintainer line. Both of them are entire coding sequences, upstream from nad3 and rps12 gene, and co-transcribed with the nad3 and rps12 genes. In addition, two molecular markers, orf256 and orf324/orf305, have been successfully converted into the SCAR markers. Subsequently, ORF256, ORF324, ORF305 protein and ORF256-M-431 fragment are predicated to contain signal peptide sequences, and ORF220 was predicated to contain signal anchor sequence. RFLP analysis results revealed that all of the molecular markers exhibited polymorphisms. Northern blot analysis indicated that the expression level of these genes in CMS line is higher than that of the maintainer line. In the mass, all of these genes are expressed lower in the leaf than that of floral organs between the CMS line and its maintainer line. Results of this study also provide some novel and useful clues to explore the biological function of these specific marker genes in the tuber mustard.
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影响因子：
3.1
作者：
Sugiyama, Y;Watase, Y;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
2.5
作者：
C. Rankin;Marc Cutright;C. Makaroff
通讯作者：
C. Rankin;Marc Cutright;C. Makaroff
影响因子：
3.8
作者：
HUISMAN, MJ;LINTHORST, HJM;CORNELISSEN, BJC
通讯作者：
CORNELISSEN, BJC
影响因子：
3.5
作者：
Mizumoto, K;Murai, K;Takumi, S
通讯作者：
Takumi, S
影响因子：
3.1
作者：
SONG, JS;HEDGCOTH, C
通讯作者：
HEDGCOTH, C