Analysis of the interactions of botanical extract combinations against the viability of prostate cancer cell lines.
Analysis of the interactions of botanical extract combinations against the viability of prostate cancer cell lines.
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DOI：
10.1093/ecam/nel001
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发表时间：
2006-03
期刊：
影响因子：
--
通讯作者：
Heber D
中科院分区：
文献类型：
--
作者：
Adams LS;Seeram NP;Hardy ML;Carpenter C;Heber D
草药通常是植物提取物的组合，被认为具有相加或协同作用。本研究的目的是比较单个植物提取物与提取物组合对前列腺细胞活力的影响。然后，我们以等辐射线的方式对植物提取物之间的相互作用进行建模。收集黄芩、冬凌草、三七、菊花、乌拉尔甘草和锯棕榈，进行分类鉴定并制备提取物。使用发光 ATP 细胞活力测定法定量前列腺细胞系中提取物对细胞活力的影响。在 22Rv1 细胞系中测试了四种最活性提取物中的两种植物提取物的组合，并使用等辐射线分析评估了它们的相互作用。除匍匐草外，每种提取物均以时间和剂量依赖性方式显着抑制前列腺细胞系的增殖。最活跃的提取物：黄芩、桑叶、乌拉尔和冬凌草作为两种提取物的组合进行了测试。黄芩和桑葚组合时是相加的，具有协同作用的趋势，而桑葚和冬凌草组合在一起是相加的。其余的两种提取物组合显示出拮抗作用。四种提取物一起使用比两两组合和单独的提取物更有效。与单独提取物相比，四种草药提取物的组合显着增强了其在测试细胞系中的活性。两种组合的不可预测性表明需要根据每种草药的预期用途仔细描述每种草药的效果。
Herbal medicines are often combinations of botanical extracts that are assumed to have additive or synergistic effects. The purpose of this investigation was to compare the effect of individual botanical extracts with combinations of extracts on prostate cell viability. We then modeled the interactions between botanical extracts in combination isobolographically. Scutellaria baicalensis, Rabdosia rubescens, Panax-pseudo ginseng, Dendranthema morifolium, Glycyrrhiza uralensis and Serenoa repens were collected, taxonomically identified and extracts prepared. Effects of the extracts on cell viability were quantitated in prostate cell lines using a luminescent ATP cell viability assay. Combinations of two botanical extracts of the four most active extracts were tested in the 22Rv1 cell line and their interactions assessed using isobolographic analysis. Each extract significantly inhibited the proliferation of prostate cell lines in a time- and dose-dependent manner except S. repens. The most active extracts, S. baicalensis, D. morifolium, G. uralensis and R. rubescens were tested as two-extract combinations. S. baicalensis and D. morifolium when combined were additive with a trend toward synergy, whereas D. morifolium and R. rubescens together were additive. The remaining two-extract combinations showed antagonism. The four extracts together were significantly more effective than the two-by-two combinations and the individual extracts alone. Combining the four herbal extracts significantly enhanced their activity in the cell lines tested compared with extracts alone. The less predictable nature of the two-way combinations suggests a need for careful characterization of the effects of each individual herb based on their intended use.
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5.1
作者：
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