Brazilian propolis mitigates impaired glucose and lipid metabolism in experimental periodontitis in mice.
Brazilian propolis mitigates impaired glucose and lipid metabolism in experimental periodontitis in mice.
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DOI：
10.1186/s12906-016-1305-8
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发表时间：
2016-08-30
影响因子：
--
通讯作者：
Yamazaki K
中科院分区：
文献类型：
--
作者：
Nakajima M;Arimatsu K;Minagawa T;Matsuda Y;Sato K;Takahashi N;Nakajima T;Yamazaki K
牙周炎已被认为是与胰岛素抵抗相关的代谢紊乱的危险因素。最近，我们已经证明，口服牙龈卟啉单胞菌，一种代表性的牙周病细菌，诱导内毒素血症通过降低肠道屏障功能加上肠道微生物群组成的变化，导致全身炎症和胰岛素抵抗。蜂胶是蜜蜂从各种植物的叶芽和树皮裂缝中收集的一种可吸收物质，可以在各种实验模型中对代谢紊乱产生积极影响。因此，在本研究中，我们的目的是澄清牙龈卟啉单胞菌管理诱导的糖和脂质代谢受损的影响。8周龄雄性C57 BL/6小鼠经口给予牙龈卟啉单胞菌菌株W83、含牙龈卟啉单胞菌的乙醇提取物粉末或溶媒。然后，我们分析了葡萄糖和脂质代谢相关基因在肝脏和脂肪组织中的表达谱。还通过鲎试剂试验评价了血清内毒素水平。此外，我们还对肝脏进行了组织学分析，并通过测量下第一磨牙的根表面积来量化牙槽骨的损失。口服牙龈卟啉单胞菌可诱导脂肪组织中改善胰岛素敏感性的基因（C1qtnf9、Irs1和Sirt1）下调，但与脂滴形成和脂质体生成相关的基因（Plin2、Acox和G6pc）上调。然而，伴随施用的P. gingivalis消除了这些不利影响。与基因表达一致，组织学分析表明，给予的顺铂抑制了牙龈卟啉单胞菌诱导的肝脂肪变性。此外，利多卡因还能抑制由牙龈卟啉单胞菌引起的血清内毒素水平的升高。与全身效应相反，骨吸收对牙槽骨丢失没有有益的影响。这些结果表明，给予地塞米松可能有效抑制牙周病细菌引起的代谢变化，增加各种全身性疾病的风险。
Periodontitis has been implicated as a risk factor for metabolic disorders associated with insulin resistance. Recently, we have demonstrated that orally administered Porphyromonas gingivalis, a representative periodontopathic bacterium, induces endotoxemia via reduced gut barrier function coupled with changes in gut microbiota composition, resulting in systemic inflammation and insulin resistance. Propolis, a resinous substance collected by honeybees from leaf buds and cracks in the bark of various plants, can positively affect metabolic disorders in various experimental models. In this study, we thus aimed to clarify the effect of propolis on impaired glucose and lipid metabolism induced by P. gingivalis administration. Eight-week-old male C57BL/6 mice were orally administered P. gingivalis strain W83, propolis ethanol extract powder with P. gingivalis, or vehicle. We then analyzed the expression profile of glucose and lipid metabolism-related genes in the liver and adipose tissues. Serum endotoxin levels were also evaluated by a limulus amebocyte lysate test. In addition, we performed histological analysis of the liver and quantified alveolar bone loss by measuring the root surface area on the lower first molar. Oral administration of P. gingivalis induced downregulation of genes that improve insulin sensitivity in adipose tissue (C1qtnf9, Irs1, and Sirt1), but upregulation of genes associated with lipid droplet formation and gluconeogenesis (Plin2, Acox, and G6pc). However, concomitant administration of propolis abrogated these adverse effects of P. gingivalis. Consistent with gene expression, histological analysis showed that administered propolis suppressed hepatic steatosis induced by P. gingivalis. Furthermore, propolis inhibited the elevation of serum endotoxin levels induced by P. gingivalis administration. Contrary to the systemic effects, propolis had no beneficial effect on alveolar bone loss. These results suggest that administration of propolis may be effective in suppressing periodontopathic bacteria-induced metabolic changes that increase the risk of various systemic diseases.
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影响因子：
4.6
作者：
Arimatsu K;Yamada H;Miyazawa H;Minagawa T;Nakajima M;Ryder MI;Gotoh K;Motooka D;Nakamura S;Iida T;Yamazaki K
通讯作者：
Yamazaki K
影响因子：
3.3
作者：
Kitamura H;Naoe Y;Kimura S;Miyamoto T;Okamoto S;Toda C;Shimamoto Y;Iwanaga T;Miyoshi I
通讯作者：
Miyoshi I
影响因子：
4.8
作者：
Bahekar, Amol Ashok;Singh, Sarabjeet;Arora, Rohit
通讯作者：
Arora, Rohit
影响因子：
3
作者：
BAKER, PJ;EVANS, RT;ROOPENIAN, DC
通讯作者：
ROOPENIAN, DC
影响因子：
2.4
作者：
Aral, Cuneyt Asim;Kesim, Servet;Yakan, Birkan
通讯作者：
Yakan, Birkan