Divergent astrovirus associated with neurologic disease in cattle.
Divergent astrovirus associated with neurologic disease in cattle.
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DOI：
10.3201/eid1909.130682
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发表时间：
2013
影响因子：
11.8
通讯作者：
Delwart E
中科院分区：
文献类型：
--
作者：
Li L;Diab S;McGraw S;Barr B;Traslavina R;Higgins R;Talbot T;Blanchard P;Rimoldi G;Fahsbender E;Page B;Phan TG;Wang C;Deng X;Pesavento P;Delwart E
利用急性神经系统疾病的年轻成年杂交牛脑组织的病毒宏基因组学，我们对一种新型星状病毒（BoAstV-NeuroS1）的全基因组进行了测序，该病毒在系统发育上与一种绵羊星状病毒相关。回顾性分析了32例病因不明的牛脑炎病例，并利用PCR和病毒RNA原位杂交检测了另外3例感染动物。病毒RNA仅限于神经系统，并在脊髓、脑干和小脑内受影响神经元的细胞质中检测到。显微镜下，病变表现为广泛的神经元坏死、小胶质细胞增生和血管周围弯曲，优先分布于灰质，最严重的是小脑和脑干，脊髓尾端的强度增加。这些结果表明，感染BoAstV-NeuroS1是牛神经系统疾病的潜在原因。
Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle.
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影响因子：
3.7
作者：
Jiang H;Holtz LR;Bauer I;Franz CJ;Zhao G;Bodhidatta L;Shrestha SK;Kang G;Wang D
通讯作者：
Wang D
影响因子：
11.8
作者：
Li L;McGraw S;Zhu K;Leutenegger CM;Marks SL;Kubiski S;Gaffney P;Dela Cruz FN Jr;Wang C;Delwart E;Pesavento PA
通讯作者：
Pesavento PA
影响因子：
9.4
作者：
Blomstrom, Anne-Lie;Widen, Frederik;Berg, Mikael
通讯作者：
Berg, Mikael
影响因子：
6.7
作者：
Victoria JG;Kapoor A;Dupuis K;Schnurr DP;Delwart EL
通讯作者：
Delwart EL
影响因子：
5
作者：
Jonassen, CM;Jonassen, TO;Grinde, B
通讯作者：
Grinde, B