The morphology and spatial arrangement of astrocytes in the optic nerve head of the mouse.
The morphology and spatial arrangement of astrocytes in the optic nerve head of the mouse.
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DOI：
10.1002/cne.22058
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发表时间：
2009-09-01
期刊：
影响因子：
--
通讯作者：
Jakobs TC
中科院分区：
文献类型：
--
作者：
Sun D;Lye-Barthel M;Masland RH;Jakobs TC
我们评估了胶质层内星形胶质细胞的形状、数量和空间分布，胶质层是视网膜和视神经交界处的一个富含星形胶质细胞的区域。主要目的是确定星形胶质细胞群体如何共同划分该区域的轴突空间。胶质纤维酸性蛋白(GFAP)标记的星形胶质细胞突起将神经节细胞轴突分隔成束，形成“胶质管”，使横切面上的视神经头的胶质构筑呈蜂窝状。利用在分离的星形胶质细胞中表达增强型绿色荧光蛋白的转基因小鼠(hGFAPpr-EGFP)研究单个星形胶质细胞的形状。在胶质板层内，星形胶质细胞呈横向排列，粗大、光滑的初级突起由胞质扩张而来。相邻星形胶质细胞突起之间的间隙在空间上对齐，形成视轴突束通过的孔。单个星形胶质细胞的突起影响深远--它们可以跨越神经的大部分宽度--并与其他近距离和远距离星形胶质细胞的解剖结构域重叠。因此，胶质层中的星形胶质细胞不会瓦解：每个星形胶质细胞参与包裹神经中大约四分之一的轴突束，每个神经胶质管包含~9个星形胶质细胞的突起。这提出了一个机制问题，在青光眼或其他神经损伤的情况下，神经胶质反应如何限制在轴突受损的有限区域。
We evaluated the shapes, numbers, and spatial distribution of astrocytes within the glial lamina, an astrocyte-rich region at the junction of the retina and optic nerve. A primary aim was to determine how the population of astrocytes, collectively, partitions the axonal space in this region. Astrocyte processes labeled with glial fibrillary acidic protein (GFAP) compartmentalize ganglion cell axons into bundles, forming “glial tubes”, and giving the glial architecture of the optic nerve head in transverse section a honeycomb appearance. The shapes of individual astrocytes were studied using transgenic mice that express enhanced green fluorescent protein in isolated astrocytes (hGFAPpr-EGFP). Within the glial lamina the astrocytes were transverse in orientation, with thick, smooth primary processes emanating from a cytoplasmic expansion of the soma. Spaces between the processes of neighboring astrocytes were spatially aligned, to form the apertures through which the bundles of optic axons pass. The processes of individual astrocytes were far-reaching – they could span most of the width of the nerve -and overlapped the anatomical domains of other near and distant astrocytes. Thus, astrocytes in the glial lamina do not tile: each astrocyte participates in ensheathing approximately one quarter of all of the axon bundles in the nerve, and each glial tube contains the processes of ~ 9 astrocytes. This raises the mechanistic question how, in glaucoma or other cases of nerve damage, the glial response can be confined to a circumscribed region where damage to axons has occurred.
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DOI：
10.1083/jcb.100.2.384
发表时间：
1985-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hatten ME
通讯作者：
Hatten ME
影响因子：
6.2
作者：
DISTLER, C;DREHER, Z;STONE, J
通讯作者：
STONE, J
影响因子：
5.3
作者：
Bushong, EA;Martone, ME;Ellisman, MH
通讯作者：
Ellisman, MH
DOI：
10.1007/bf01184072
发表时间：
1994-08-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
BUTT, AM;DUNCAN, A;BERRY, M
通讯作者：
BERRY, M
影响因子：
--
作者：
Emsley, Jason G.;Macklis, Jeffrey D.
通讯作者：
Macklis, Jeffrey D.