The extracellular matrix of Volvox carteri: molecular structure of the cellular compartment.

The extracellular matrix of Volvox carteri: molecular structure of the cellular compartment.
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DOI:
10.1083/jcb.109.6.3493
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发表时间:
1989-12
期刊:
The Journal of cell biology
影响因子:
--
通讯作者:
Sumper M
Sumper M
中科院分区:
其他
文献类型:
--
作者:
Ertl H;Mengele R;Wenzl S;Engel J;Sumper M

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团藻的细胞外基质(ECM)含有不溶性纤维层,其在一定距离处包围单个细胞以形成连续的细胞区室。使用免疫学技术,我们确定了硫酸化的表面糖蛋白(SSG 185)作为单体前体的ECM内的这个子结构。SSG 185多肽链的一级结构来源于cDNA和基因组DNA。该蛋白质的一个中心结构域长80个氨基酸残基,几乎完全由羟脯氨酸残基组成。共价连接到SSG 185的高度硫酸化多糖的化学结构已通过全甲基化分析确定。如通过EM所揭示的,SSG 185是具有从其中心区域突出的21 nm长的多糖链的棒状分子。讨论了SSG185单体间交联的化学性质.
The extracellular matrix (ECM) of Volvox contains insoluble fibrous layers that surround individual cells at a distance to form contiguous cellular compartments. Using immunological techniques, we identified a sulfated surface glycoprotein (SSG 185) as the monomeric precursor of this substructure within the ECM. The primary structure of the SSG 185 poly-peptide chain has been derived from cDNA and genomic DNA. A central domain of the protein, 80 amino acid residues long, consists almost exclusively of hydroxyproline residues. The chemical structure of the highly sulfated polysaccharide covalently attached to SSG 185 has been determined by permethylation analysis. As revealed by EM, SSG 185 is a rod-shaped molecule with a 21-nm-long polysaccharide strand protruding from its central region. The chemical nature of the cross- links between SSG 185 monomers is discussed.
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发表时间: 1985-10
期刊: The Journal of cell biology
影响因子: --
作者:
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