The whole genomic analysis of orf virus strain HN3/12 isolated from Henan province, central China.
The whole genomic analysis of orf virus strain HN3/12 isolated from Henan province, central China.
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河南省Orf病毒HN3/12株全基因组分析
DOI：
10.1186/s12917-017-1178-1
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发表时间：
2017-08-18
影响因子：
2.6
通讯作者：
Hao W
中科院分区：
文献类型：
--
作者：
Chen H;Li W;Kuang Z;Chen D;Liao X;Li M;Luo S;Hao W
背景奥尔夫病毒（ORFV）是奥尔夫病毒的病原体，奥尔夫病毒是一种全球性的急性脓疱性传染性疾病，影响绵羊、山羊和人类，并在全球范围内分布。目前，已报道了来自中国南方福建省的4株ORFV和来自中国东北吉林省的1株NA1/11毒株的基因组分析。然而，对中国中部地区ORFV毒株的基因组信息知之甚少。结果从最近在中国中部河南省的一个羊群中暴发的ORFV病毒株（HN3/12）中分离出来并在羊胎鼻甲（OFTu）细胞中培养。根据011和059基因的DNA序列，通过PCR验证该菌株为HN3/12。该分离株的整个基因组序列由下一代测序技术确定。为了确定HN3/12毒株的遗传特征，对HN3/12毒株的011和059基因进行系统发育分析以及氨基酸序列比对，并与参考副痘病毒毒株进行比较。结论HN3/12基因组长度为136,643 bp，包含63.67% G + C，编码132个推定基因。 011和059核苷酸序列的系统发育分析表明该病毒株与NA1/11分离株相似。同源性分析表明HN3/12与已发表的ORFV毒株在氨基酸水平上具有93%至98%的同一性。当HN3/12和4个福建ORFV毒株之间的开放阅读框（ORF）比对时，大多数它们的同一性大于90％，只有少数低于60％。 HN3/12 整个基因组序列的可用性有助于我们了解 ORFV 的遗传多样性，并提供新的见解。
BackgroundThe Orf virus (ORFV) is the causative agent of orf, a globally-occurring, acute, pustular, contagious disease affecting sheep, goats and humans with a worldwide distribution. Currently, the genomic analysis of four ORFV strains from the Fujian province in southern China and a NA1/11 strain isolated from the Jilin province in northeast China have been reported. However, little is known about the genomic information of ORFV strains from central China.ResultsFrom a recent outbreak in a sheep herd in the Henan province of central China, a novel ORFV strain (HN3/12) was isolated and cultured in ovine fetal turbinate (OFTu) cells. The strain was identified as HN3/12 and verified by PCR based on the DNA sequences of011and059genes. The whole genomic sequence of this isolate was determined by Next Generation Sequencing technology. To determine the genetic characteristics of the HN3/12 strain, phylogenetic analysis of the 011 and 059 genes and amino acid sequence alignment of the HN3/12 strain were performed and compared with reference parapoxvirus strains.ConclusionsThe HN3/12 genome is 136,643 bp in length, contains 63.67% G + C and encodes 132 putative genes. Phylogenetic analysis of the 011 and 059 nucleotide sequences showed that this viral strain was similar to the NA1/11 isolate. The homology analysis indicates that HN3/12 has 93% to 98% identity with published ORFV strains at amino acid level. When open reading frames (ORFs) were aligned among the HN3/12 and four Fujian ORFV strains, most of them have identities greater than 90% and only a few less than 60%. The availability of the whole genomic sequence of HN3/12 aids in our understanding of, and provides new insights into, the genetic diversity of ORFV.
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影响因子：
4.8
作者：
Gelaye E;Achenbach JE;Jenberie S;Ayelet G;Belay A;Yami M;Loitsch A;Grabherr R;Diallo A;Lamien CE
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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