Taxonomic variations in the gut microbiome of gout patients with and without tophi might have a functional impact on urate metabolism.
Taxonomic variations in the gut microbiome of gout patients with and without tophi might have a functional impact on urate metabolism.
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DOI：
10.1186/s10020-021-00311-5
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发表时间：
2021-05-24
期刊：
影响因子：
--
通讯作者：
Martínez-Nava GA
中科院分区：
文献类型：
--
作者：
Méndez-Salazar EO;Vázquez-Mellado J;Casimiro-Soriguer CS;Dopazo J;Çubuk C;Zamudio-Cuevas Y;Francisco-Balderas A;Martínez-Flores K;Fernández-Torres J;Lozada-Pérez C;Pineda C;Sánchez-González A;Silveira LH;Burguete-García AI;Orbe-Orihuela C;Lagunas-Martínez A;Vazquez-Gomez A;López-Reyes A;Palacios-González B;Martínez-Nava GA
评估痛风患者（有和无痛风石形成）肠道微生物组的分类组成，并预测可能影响尿酸盐代谢的细菌功能。对来自有和没有痛风石的痛风患者（分别为n = 33和n = 25）的粪便样品的细菌16 S rRNA基因的高变V3-V4区进行测序，并与来自53名健康对照的粪便样品进行比较。我们利用生物信息学探索了预测功能谱，以确定分类学和代谢途径的差异。我们确定了一个微生物组，其特征是与对照组相比，无痛风石的痛风患者中最低丰度和较高丰度的Phascolarctobacterium，Bacteroides，Akkermansia和Ruminococcus_gavus_group属。变形菌门和土方肠志贺菌属在痛风石患者比对照组更丰富。与痛风组相比，倍数变化分析检测到健康对照中富集的9个属（双歧杆菌属、丁酸球菌属、振荡杆菌属、瘤胃球菌属_UCG_010、毛螺菌属_ND2007_组、嗜血杆菌属、瘤胃球菌属_1、梭菌属_sensu_stricto_1和瘤胃球菌属_UGC_013）。我们发现，两组痛风的核心微生物群共有粪拟杆菌、粪拟杆菌ATCC 43183和粪拟杆菌DSM 17136。这些细菌可能执行与一碳代谢、核苷酸结合、氨基酸生物合成和嘌呤生物合成相关的功能。最后，我们观察到尿酸盐合成、降解和消除的关键细菌酶的差异。我们的研究结果表明，痛风患者的肠道微生物组的分类变化与痛风石和没有痛风石可能有功能的影响尿酸盐代谢。在线版本包含补充材料，可通过10.1186/s10020-021-00311-5获得。
To evaluate the taxonomic composition of the gut microbiome in gout patients with and without tophi formation, and predict bacterial functions that might have an impact on urate metabolism. Hypervariable V3–V4 regions of the bacterial 16S rRNA gene from fecal samples of gout patients with and without tophi (n = 33 and n = 25, respectively) were sequenced and compared to fecal samples from 53 healthy controls. We explored predictive functional profiles using bioinformatics in order to identify differences in taxonomy and metabolic pathways. We identified a microbiome characterized by the lowest richness and a higher abundance of Phascolarctobacterium, Bacteroides, Akkermansia, and Ruminococcus_gnavus_group genera in patients with gout without tophi when compared to controls. The Proteobacteria phylum and the Escherichia-Shigella genus were more abundant in patients with tophaceous gout than in controls. Fold change analysis detected nine genera enriched in healthy controls compared to gout groups (Bifidobacterium, Butyricicoccus, Oscillobacter, Ruminococcaceae_UCG_010, Lachnospiraceae_ND2007_group, Haemophilus, Ruminococcus_1, Clostridium_sensu_stricto_1, and Ruminococcaceae_UGC_013). We found that the core microbiota of both gout groups shared Bacteroides caccae, Bacteroides stercoris ATCC 43183, and Bacteroides coprocola DSM 17136. These bacteria might perform functions linked to one-carbon metabolism, nucleotide binding, amino acid biosynthesis, and purine biosynthesis. Finally, we observed differences in key bacterial enzymes involved in urate synthesis, degradation, and elimination. Our findings revealed that taxonomic variations in the gut microbiome of gout patients with and without tophi might have a functional impact on urate metabolism. The online version contains supplementary material available at 10.1186/s10020-021-00311-5.
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