Adverse Effect of TiO2 Nanoparticles on Antioxidant System and Antitumor Activities of Macroalgae Gracilaria lemaneiformis
Adverse Effect of TiO2 Nanoparticles on Antioxidant System and Antitumor Activities of Macroalgae Gracilaria lemaneiformis
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纳米TiO2颗粒对龙须菜抗氧化系统和抗肿瘤活性的不利影响
DOI：
10.1007/s11802-019-3819-4
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发表时间：
2019-10
影响因子：
1.6
通讯作者：
Zhao Ling
中科院分区：
文献类型：
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作者：
Liu Jie;Yin Pinghe;Zhao Ling
近年来，二氧化钛纳米粒子（nano-TiO2）的广泛应用引起了全球对其对水生生物的不利影响的关注。纳米颗粒可能进入水生环境，危害藻类的生长。本文研究了纳米tio2浓度对0、5、10、20、40 mg L-1纳米tio2对海洋大型藻类扁豆藻光合特性、抗氧化系统和抗肿瘤活性的影响。结果表明，纳米tio2暴露后，毛竹光合色素含量明显降低，抗氧化防御系统受到明显干扰。与对照组相比，暴露于纳米tio2 15天后，丙二醛的最大水平增加了3.3倍。超氧化物歧化酶和过氧化氢酶活性明显提高1.10 ~ 2.24倍。水提物对HepG2肝癌细胞增殖具有抑制作用，对48 h细胞的抑制作用超过35%。但纳米tio2的毒性会抑制AEGL的抗癌活性。当纳米tio2暴露于lemaneiformis时，lemaneiformis水提液对HepG2细胞的抑制作用、活性氧产生、细胞凋亡、周期阻滞作用显著减弱，且与纳米tio2暴露浓度呈线性相关。这些结果进一步证实了纳米tio2可以抑制lemaneiformis的抗癌活性。本研究结果表明，巨藻可作为纳米tio2污染物的有效生物标志物，并可作为评价海洋生态系统中污染物增加的生态效应的有用指标。
Recently, the widespread use of TiO2 nanoparticles (nano-TiO2) has attracted the global concern regarding their adverse effects to aquatic organisms. Nanoparticles may get to the aquatic environments and harm the growth of algae. This paper presents the studies of nano-TiO2 concentrations influence on the photosynthetic characteristics, antioxidant system and antitumor activity of marine macroalgae Gracilaria lemaneiformis after exposed to 0, 5, 10, 20 or 40 mg L-1 of nano-TiO2. Results showed that the photosynthetic pigment content of G. lemaneiformis obviously decreased and the antioxidant defense system was significantly disturbed after exposure to nano-TiO2. Compared with control, the maximum level of malondialdehyde showed 3.3-fold increase for 15 days exposure to nano-TiO2. The activity of superoxide dismutase and catalase enhanced obviously by 1.10–2.24-fold. The aqueous extract of G. lemaneiformis (AEGL) possesses anticancer activity against HepG2 liver cancer cells proliferation and the 48 h-cell inhibition exceeds 35%. But the toxicity of nano-TiO2 would inhibit the anticancer activity of AEGL. When G. lemaneiformis was exposed to nano-TiO2, the effect of the aqueous extract of G. lemaneiformis on the cell inhibition, reactive oxygen species production, apoptosis, cycle arrest of HepG2 cells significantly lessened, and which were linearly associated with nano-TiO2 exposure concentration. Those results further confirmed that nano-TiO2 can inhibit the anticancer activity of G. lemaneiformis. The results in this work imply that macroalgae can be an effective biomarker of nano-TiO2 contaminant, and can be useful indicator to evaluate ecological effects of increasing pollutants in marine ecosystem.
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影响因子：
--
作者：
K. Zandi;S. Tajbakhsh;I. Nabipour;Z. Rastian;F. Yousefi;S. Sharafian
通讯作者：
K. Zandi;S. Tajbakhsh;I. Nabipour;Z. Rastian;F. Yousefi;S. Sharafian
影响因子：
6
作者：
E. Marinho-Soriano;S. O. Nunes;M. Carneiro;D. C. Pereira
通讯作者：
E. Marinho-Soriano;S. O. Nunes;M. Carneiro;D. C. Pereira
影响因子：
6.1
作者：
Huang, HL;Wu, SL;Chen, YJ
通讯作者：
Chen, YJ
影响因子：
2.2
作者：
J. Patra;S. Rath;K. Jena;V. Rathod;H. Thatoi
通讯作者：
J. Patra;S. Rath;K. Jena;V. Rathod;H. Thatoi
影响因子：
3.3
作者：
L. Mendes;L. Zambotti‐Villela;P. Colepicolo;E. Marinho-Soriano;Cassius Vinicius Stevani;N. Yokoya
通讯作者：
L. Mendes;L. Zambotti‐Villela;P. Colepicolo;E. Marinho-Soriano;Cassius Vinicius Stevani;N. Yokoya