Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya.
Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya.
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DOI：
10.1038/s41598-021-88327-z
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发表时间：
2021-04-23
影响因子：
4.6
通讯作者：
Ouma C
中科院分区：
文献类型：
--
作者：
Akoko JM;Pelle R;Lukambagire AS;Machuka EM;Nthiwa D;Mathew C;Fèvre EM;Bett B;Cook EAJ;Othero D;Bonfoh B;Kazwala RR;Shirima G;Schelling E;Halliday JEB;Ouma C
布鲁氏菌病是由布鲁氏菌属的几个物种引起的一种人畜共患疾病，影响全世界的人类和动物物种。关于在肯尼亚不同宿主中传播的布鲁氏菌的信息在很大程度上是未知的，因此限制了有针对性的控制策略的采用。本研究在肯尼亚的多宿主牲畜种群中进行，以检测循环的布鲁氏菌种属并评估宿主-病原体关联的证据。从肯尼亚纳罗克县和马尔萨比特县的228头牛、162只山羊、158只绵羊、49头骆驼和257名人类中采集血清样本。获得了采样牲畜的年龄、位置和流产或胎盘滞留史信息。还收集了人类参与者的年龄、性别和居住地数据。所有样本均使用属级实时PCR检测试剂盒进行检测，引物特异性针对用于检测布鲁氏菌的IS 711和bcsp 31靶标。所有属阳性样本（两种靶标均为阳性）均进一步采用Alk B和BMEI 1162靶标（对B具有特异性）的形态分析进行检测。流产和B. melitensis，分别。还采用虎红试验（RBT）检测了足量聚集至577的样本。共有199份（33.3%）牲畜和99份（38.5%）人类样本对布鲁氏菌属检测呈阳性。RBT阳性结果可预测动物布鲁氏菌PCR阳性状态（OR = 8.3，95%CI 4.0-17.1）。与其他年龄组相比，21-40岁的人群布鲁氏菌PCR阳性的几率更高（OR = 2.8，95% CI 1.2-6.6）。对不同种布鲁氏菌的检测数据表明，B.牛（OR = 2.3，95%CI 1.1-4.6）和骆驼（OR = 2.9，95%CI 1.3-6.3）流产率较高，而B.绵羊和山羊中检出羊种的比例较高（OR = 3.6，95% CI 2.0-6.7）和（OR = 1.7，95% CI 1.0-3.1）。都是B。流产和B.在人类和多种家畜宿主物种中检测到羊种布氏菌DNA，表明这些物种在不同宿主之间交叉传播。在人类中检测到这两种人畜共患布鲁氏菌，进一步强调了针对多个宿主物种，特别是多宿主牲畜种群的“一个健康”预防战略的重要性。
Brucellosis, caused by several species of the genus Brucella, is a zoonotic disease that affects humans and animal species worldwide. Information on the Brucella species circulating in different hosts in Kenya is largely unknown, thus limiting the adoption of targeted control strategies. This study was conducted in multi-host livestock populations in Kenya to detect the circulating Brucella species and assess evidence of host–pathogen associations. Serum samples were collected from 228 cattle, 162 goats, 158 sheep, 49 camels, and 257 humans from Narok and Marsabit counties in Kenya. Information on age, location and history of abortion or retained placenta were obtained for sampled livestock. Data on age, gender and location of residence were also collected for human participants. All samples were tested using genus level real-time PCR assays with primers specific for IS711 and bcsp31 targets for the detection of Brucella. All genus positive samples (positive for both targets) were further tested with a speciation assay for AlkB and BMEI1162 targets, specific for B. abortus and B. melitensis, respectively. Samples with adequate quantities aggregating to 577 were also tested with the Rose Bengal Test (RBT). A total of 199 (33.3%) livestock and 99 (38.5%) human samples tested positive for genus Brucella. Animal Brucella PCR positive status was positively predicted by RBT positive results (OR = 8.3, 95% CI 4.0–17.1). Humans aged 21–40 years had higher odds (OR = 2.8, 95% CI 1.2–6.6) of being Brucella PCR positive compared to the other age categories. The data on detection of different Brucella species indicates that B. abortus was detected more often in cattle (OR = 2.3, 95% CI 1.1–4.6) and camels (OR = 2.9, 95% CI 1.3–6.3), while B. melitensis was detected more in sheep (OR = 3.6, 95% CI 2.0–6.7) and goats (OR = 1.7, 95% CI 1.0–3.1). Both B. abortus and B. melitensis DNA were detected in humans and in multiple livestock host species, suggesting cross-transmission of these species among the different hosts. The detection of these two zoonotic Brucella species in humans further underpins the importance of One Health prevention strategies that target multiple host species, especially in the multi-host livestock populations.
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DOI：
10.4102/jsava.v88i0.1381
发表时间：
2017-01-01
影响因子：
0.9
作者：
Chisi, Songelwayo L.;Marageni, Yoanda;van Heerden, Henriette
通讯作者：
van Heerden, Henriette
影响因子：
1.7
作者：
Ahasan, Md. Shamim;Rahman, Md. Siddiqur;Berkvens, Dirk
通讯作者：
Berkvens, Dirk
影响因子：
2.2
作者：
Kalleshamurthy, Triveni;Skariah, Somy;Shome, Rajeswari
通讯作者：
Shome, Rajeswari
影响因子：
2.6
作者：
Godfroid, J.;Scholz, H. C.;Letesson, J. -J.
通讯作者：
Letesson, J. -J.
影响因子：
3.8
作者：
Kairu-Wanyoike, Salome;Nyamwaya, Doris;Bett, Bernard
通讯作者：
Bett, Bernard