Self-assembling peptide-laden electrospun scaffolds for guided mineralized tissue regeneration.
Self-assembling peptide-laden electrospun scaffolds for guided mineralized tissue regeneration.
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DOI：
10.1016/j.dental.2022.09.011
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发表时间：
2022-11
期刊：
影响因子：
5
通讯作者：
Bottino, Marco C.
中科院分区：
文献类型：
--
作者：
Araujo, Isaac J. de Souza;Ferreira, Jessica A.;Daghrery, Arwa;Ribeiro, Juliana S.;Castilho, Miguel;Puppin-Rontani, Regina M.;Bottino, Marco C.
电纺支架是一种多功能生物材料平台，可模拟天然组织细胞外基质的纤维结构，并促进再生治疗中生物分子的掺入。自组装肽 P11-4 已成为一种有前景的诱导矿化策略；然而，P11-4的应用主要用于修复牙釉质上的早期龋齿病变。在这里，为了研究 P11-4 对骨再生的功效，开发了聚合物静电纺丝支架，然后将不同浓度的 P11-4 物理吸附在支架上。将负载 P11-4 和原始（不含 P11-4）的电纺支架浸入模拟体液中，并通过 SEM 鉴定矿物质沉淀。分别通过 FTIR 和 XRD 分析官能团和晶相。使用来自人类脱落乳牙的干细胞进行细胞相容性、矿化和基因表达测定。为了研究负载 P11-4 的支架诱导体内矿化的潜力，使用了已建立的大鼠颅骨临界尺寸缺陷模型。结果。我们成功合成了纳米纤维（纤维直径约 500 nm）支架，并观察到 P11-4 的功能化不会影响纤维的直径。 SEM 图像显示矿物沉淀，而 FTIR 和 XRD 证实了负载 P11-4 的支架的磷灰石状形成和结晶。此外，与原始支架相比，负载 P11-4 的支架具有细胞相容性，高度刺激细胞介导的矿物质沉积，并上调矿化相关基因的表达。与原始 PCL 相比，负载 P11-4 的支架在 8 周后导致体内骨再生增强。用 P11-4 功能化的电纺支架是诱导颅颌面复合体矿化组织再生的有前途的策略。
Electrospun scaffolds are a versatile biomaterial platform to mimic fibrillar structure of native tissues extracellular matrix, and facilitate the incorporation of biomolecules for regenerative therapies. Self-assembling peptide P11-4 has emerged as a promising strategy to induce mineralization; however, P11-4 application has been mostly addressed for early caries lesions repair on dental enamel. Here, to investigate P11-4’s efficacy on bone regeneration, polymeric electrospun scaffolds were developed, and then distinct concentrations of P11-4 were physically adsorbed on the scaffolds. P11-4-laden and pristine (P11-4-free) electrospun scaffolds were immersed in simulated body fluid and mineral precipitation identified by SEM. Functional groups and crystalline phases were analyzed by FTIR and XRD, respectively. Cytocompatibility, mineralization, and gene expression assays were conducted using stem cells from human exfoliated deciduous teeth. To investigate P11-4-laden scaffolds potential to induce in vivo mineralization, an established rat calvaria critical-size defect model was used. Results. We successfully synthesized nanofibrous (~ 500 nm fiber diameter) scaffolds and observed that functionalization with P11-4 did not affect the fibers’ diameter. SEM images indicated mineral precipitation, while FTIR and XRD confirmed apatite-like formation and crystallization for P11-4-laden scaffolds. In addition, P11-4-laden scaffolds were cytocompatible, highly stimulated cell-mediated mineral deposition, and upregulated the expression of mineralization-related genes compared to pristine scaffolds. P11-4-laden scaffolds led to enhanced in vivo bone regeneration after 8 weeks compared to pristine PCL. Electrospun scaffolds functionalized with P11-4 are a promising strategy for inducing mineralized tissues regeneration in the craniomaxillofacial complex.
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影响因子：
5.7
作者：
El-Sayed B;Davies RPW;El-Zehery RR;Ibrahim FM;Grawish ME;Kirkham J;El-Gendy R
通讯作者：
El-Gendy R
影响因子：
15
作者：
Aggeli, A;Bell, M;Boden, N
通讯作者：
Boden, N
影响因子：
64.8
作者：
Aggeli, A;Bell, M;Radford, SE
通讯作者：
Radford, SE
影响因子：
2.1
作者：
Barco, A.;Ingham, E.;Davies, R. P. W.
通讯作者：
Davies, R. P. W.
影响因子：
7.6
作者：
Kind, L.;Stevanovic, S.;Pieles, U.
通讯作者：
Pieles, U.