Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid.
Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid.
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DOI：
10.1007/s00726-014-1762-7
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发表时间：
2014-09
期刊：
影响因子：
3.5
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Ruan, Zheng;Yang, Yuhui;Zhou, Yan;Wen, Yanmei;Ding, Sheng;Liu, Gang;Wu, Xin;Liao, Peng;Deng, Zeyuan;Assaad, Houssein;Wu, Guoyao;Yin, Yulong
本研究旨在研究补充绿原酸（CGA）对大鼠血清和肝脏代谢组的影响。大鼠每天接受 CGA（60 毫克/千克体重）或蒸馏水（对照）灌胃，持续 4 周。测量生长性能、血清生化特征和肝脏形态。此外，还通过基于高分辨率 1H 核磁共振的代谢组学和多变量统计分析了血清和肝组织提取物的代谢组。 CGA 不影响大鼠生长性能、血清生化特征或肝脏形态。然而，补充CGA降低了乳酸盐、丙酮酸盐、琥珀酸盐、柠檬酸盐、β-羟基丁酸盐和乙酰乙酸盐的血清浓度，同时增加了甘氨酸的血清浓度和谷胱甘肽的肝脏浓度。这些结果表明，补充 CGA 会扰乱大鼠的能量和氨基酸代谢。我们认为血清中的甘氨酸和谷胱甘肽可能是 CGA 对体内氮代谢生物学特性的有用生物标志物。
This study was conducted to investigate effects of chlorogenic acid (CGA) supplementation on serum and hepatic metabolomes in rats. Rats received daily intragastric administration of either CGA (60 mg/kg body weight) or distilled water (control) for 4 weeks. Growth performance, serum biochemical profiles, and hepatic morphology were measured. Additionally, serum and liver tissue extracts were analyzed for metabolomes by high-resolution 1H nuclear magnetic resonance-based metabolomics and multivariate statistics. CGA did not affect rat growth performance, serum biochemical profiles, or hepatic morphology. However, supplementation with CGA decreased serum concentrations of lactate, pyruvate, succinate, citrate, β-hydroxybutyrate and acetoacetate, while increasing serum concentrations of glycine and hepatic concentrations of glutathione. These results suggest that CGA supplementation results in perturbation of energy and amino acid metabolism in rats. We suggest that glycine and glutathione in serum may be useful biomarkers for biological properties of CGA on nitrogen metabolism in vivo.
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影响因子：
3.8
作者：
AlGamdi, Noura;Mullen, William;Crozier, Alan
通讯作者：
Crozier, Alan
影响因子：
2.1
作者：
Diaz-Flores, Margarita;Cruz, Miguel;Hernandez-Saavedra, Daniel
通讯作者：
Hernandez-Saavedra, Daniel
DOI：
10.1152/ajpregu.00159.2004
发表时间：
2004-12-01
影响因子：
2.8
作者：
El Hafidi, M;Pérez, I;Baños, G
通讯作者：
Baños, G
DOI：
10.1046/j.1439-0396.2003.00432.x
发表时间：
2003-06-01
影响因子：
2.7
作者：
He, ML;Wang, YZ;Rambeck, WA
通讯作者：
Rambeck, WA
影响因子：
3.5
作者：
Dai, Zhao-Lai;Li, Xi-Long;Zhu, Wei-Yun
通讯作者：
Zhu, Wei-Yun