3D printing of composite calcium phosphate and collagen scaffolds for bone regeneration.
3D printing of composite calcium phosphate and collagen scaffolds for bone regeneration.
复制标题
DOI：
10.1016/j.biomaterials.2014.01.064
复制
发表时间：
2014-04
期刊：
影响因子：
14
通讯作者：
Awad HA
中科院分区：
文献类型：
--
作者：
Inzana JA;Olvera D;Fuller SM;Kelly JP;Graeve OA;Schwarz EM;Kates SL;Awad HA
磷酸钙支架的低温 3D 打印在制造合成骨移植替代品方面具有广阔的前景，其性能优于传统技术。许多设计参数（例如粘合剂溶液特性）尚未优化，以确保最大的生物相容性和骨传导性以及足够的机械性能。本研究将磷酸基粘合剂溶液浓度调整为 8.75 wt%，以最大限度地提高细胞相容性和机械强度，并补充 Tween 80 以改善打印。为了进一步增强配方，将胶原蛋白溶解到粘合剂溶液中以制造胶原蛋白-磷酸钙复合材料。通过生理热处理和 Tween 80 分别降低粘度和表面张力，实现了胶原蛋白溶液的可靠热喷墨打印。在粘合剂溶液中补充 1-2 wt% 胶原蛋白可显着提高最大弯曲强度和细胞活力。为了评估骨愈合性能，我们将 3D 打印支架植入尺寸临界的小鼠股骨缺损中，持续 9 周。植入物被证实具有骨传导性，新骨生长结合了降解支架材料。总之，本研究证明了 3D 打印磷酸钙支架的材料参数的优化，以及通过喷墨打印体积胶原掺入来增强材料性能。
Low temperature 3D printing of calcium phosphate scaffolds holds great promise for fabricating synthetic bone graft substitutes with enhanced performance over traditional techniques. Many design parameters, such as the binder solution properties, have yet to be optimized to ensure maximal biocompatibility and osteoconductivity with sufficient mechanical properties. This study tailored the phosphoric acid-based binder solution concentration to 8.75 wt% to maximize cytocompatibility and mechanical strength, with a supplementation of Tween 80 to improve printing. To further enhance the formulation, collagen was dissolved into the binder solution to fabricate collagen-calcium phosphate composites. Reducing the viscosity and surface tension through a physiologic heat treatment and Tween 80, respectively, enabled reliable thermal inkjet printing of the collagen solutions. Supplementing the binder solution with 1–2 wt% collagen significantly improved maximum flexural strength and cell viability. To assess the bone healing performance, we implanted 3D printed scaffolds into a critically sized murine femoral defect for 9 weeks. The implants were confirmed to be osteoconductive, with new bone growth incorporating the degrading scaffold materials. In conclusion, this study demonstrates optimization of material parameters for 3D printed calcium phosphate scaffolds and enhancement of material properties by volumetric collagen incorporation via inkjet printing.
登录
查看更多内容
影响因子：
9.7
作者：
Butscher, Andre;Bohner, Marc;Mueller, Ralph
通讯作者：
Mueller, Ralph
影响因子：
19
作者：
Gbureck, Uwe;Hoezel, Tanja;Barralet, Jake E.
通讯作者：
Barralet, Jake E.
DOI：
10.1007/s10047-006-0347-y
发表时间：
2006-01-01
期刊：
Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs
影响因子：
--
作者：
Igawa, Kazuyo;Mochizuki, Manabu;Sasaki, Nobuo
通讯作者：
Sasaki, Nobuo
影响因子：
3
作者：
Manassero, Mathieu;Viateau, Veronique;Petite, Herve
通讯作者：
Petite, Herve
影响因子：
14
作者：
Wojtowicz, Abigail M.;Shekaran, Asha;Oest, Megan E.;Dupont, Kenneth M.;Templeman, Kellie L.;Hutmacher, Dietmar W.;Guldberg, Robert E.;Garcia, Andres J.
通讯作者：
Garcia, Andres J.