Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules.
Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules.
复制标题
DOI：
10.1038/s41598-017-09201-5
复制
发表时间：
2017-08-17
影响因子：
4.6
通讯作者：
Shim JH
中科院分区：
文献类型：
--
作者：
Ahn G;Min KH;Kim C;Lee JS;Kang D;Won JY;Cho DW;Kim JY;Jin S;Yun WS;Shim JH
三维（3D）细胞打印系统允许多个细胞在3D构建体中的受控和精确沉积。水凝胶材料由于其安全封装活细胞的能力而被广泛用作可打印的生物墨水。然而，基于水凝胶的生物墨水对于细胞打印具有缺点，例如不适当的交联和液体状流变性质，这阻碍了精确的3D成形。因此，在这项研究中，我们研究了各种因素（例如生物墨水浓度，粘度和交联程度）对细胞打印的影响，并建立了一种新的3D细胞打印系统，该系统配备了加热模块，用于精确堆叠基于脱细胞外基质（dECM）的3D细胞负载结构。由于从天然组织中分离的pH调节的生物墨水在37 °C下安全地凝胶化，因此我们的加热系统通过在打印期间实现同时凝胶化来促进dECM生物墨水的精确堆叠。我们观察到更大的印刷适性相比，一个非加热系统。这些结果通过力学测试和3D结构堆叠分析得到证实。我们还证实，我们的加热系统不会在打印的细胞中引起负面影响，例如细胞死亡。总之，这些结果有望将3D生物打印应用于组织工程和药物开发。
Three-dimensional (3D) cell printing systems allow the controlled and precise deposition of multiple cells in 3D constructs. Hydrogel materials have been used extensively as printable bioinks owing to their ability to safely encapsulate living cells. However, hydrogel-based bioinks have drawbacks for cell printing, e.g. inappropriate crosslinking and liquid-like rheological properties, which hinder precise 3D shaping. Therefore, in this study, we investigated the influence of various factors (e.g. bioink concentration, viscosity, and extent of crosslinking) on cell printing and established a new 3D cell printing system equipped with heating modules for the precise stacking of decellularized extracellular matrix (dECM)-based 3D cell-laden constructs. Because the pH-adjusted bioink isolated from native tissue is safely gelled at 37 °C, our heating system facilitated the precise stacking of dECM bioinks by enabling simultaneous gelation during printing. We observed greater printability compared with that of a non-heating system. These results were confirmed by mechanical testing and 3D construct stacking analyses. We also confirmed that our heating system did not elicit negative effects, such as cell death, in the printed cells. Conclusively, these results hold promise for the application of 3D bioprinting to tissue engineering and drug development.
登录
查看更多内容
影响因子：
3
作者：
Choi, Young Chan;Choi, Ji Suk;Cho, Yong Woo
通讯作者：
Cho, Yong Woo
影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
2.8
作者：
Lee, Vivian K.;Lanzi, Alison M.;Ngo, Haygan;Yoo, Seung-Schik;Vincent, Peter A.;Dai, Guohao
通讯作者：
Dai, Guohao
影响因子：
14
作者：
Lee, Wonhye;Debasitis, Jason Cushing;Yoo, Seung-Schik
通讯作者：
Yoo, Seung-Schik
影响因子：
4.2
作者：
Ma, XH;Noishiki, Y;Matsumoto, A
通讯作者：
Matsumoto, A