Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs.
Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs.
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DOI：
10.1002/jbio.201700391
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发表时间：
2018-09
影响因子：
2.8
通讯作者：
Marcu L
中科院分区：
文献类型：
--
作者：
Alfonso-Garcia A;Shklover J;Sherlock BE;Panitch A;Griffiths LG;Marcu L
为了更有效地控制发育参数，需要能够监测组织工程构建体随时间的成熟情况的新技术。在此，报道了通过单个光纤接口实现的无标记荧光寿命成像（FLIm）方法，用于血管生物材料的无损原位评估。在 7 天的培养期间，在支架的浆液侧和纤维侧（2 个不同的细胞外基质生态位）上评估了具有内皮细胞和间充质干细胞的去除抗原的牛心包支架的再细胞化过程。结果表明，荧光寿命成功地报告了从细胞外基质荧光中解析出的细胞存在。对于两种细胞类型，浆液侧的再细胞化过程比纤维侧更快，并且在去除抗原的牛心包上，内皮细胞比间充质干细胞扩增得更快。基于纤维的 FLIm 通过对腔内血管生物材料进行原位成像，有可能成为评估组织成熟度的无损工具。
New techniques able to monitor the maturation of tissue engineered constructs over time are needed for a more efficient control of developmental parameters. Here, a label-free fluorescence lifetime imaging (FLIm) approach implemented through a single fiber-optic interface is reported for nondestructive in situ assessment of vascular biomaterials. Recellularization processes of antigen removed bovine pericardium scaffolds with endothelial cells and mesenchymal stem cells were evaluated on the serous and the fibrous sides of the scaffolds, 2 distinct extracellular matrix niches, over the course of a 7 day culture period. Results indicated that fluorescence lifetime successfully report cell presence resolved from extracellular matrix fluorescence. The recellularization process was more rapid on the serous side than on the fibrous side for both cell types, and endothelial cells expanded faster than mesenchymal stem cells on antigen-removed bovine pericardium. Fiber-based FLIm has the potential to become a nondestructive tool for the assessment of tissue maturation by allowing in situ imaging of intraluminal vascular biomaterials.
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影响因子：
2.9
作者：
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通讯作者：
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通讯作者：
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影响因子：
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作者：
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