A photo-multiplier tube-based hybrid MRI and frequency domain fluorescence tomography system for small animal imaging.
A photo-multiplier tube-based hybrid MRI and frequency domain fluorescence tomography system for small animal imaging.
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DOI：
10.1088/0031-9155/56/15/007
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发表时间：
2011-08-07
影响因子：
3.5
通讯作者：
Gulsen G
中科院分区：
文献类型：
--
作者：
Lin Y;Ghijsen MT;Gao H;Liu N;Nalcioglu O;Gulsen G
荧光层析成像(FT)是一种很有前途的分子成像技术，它可以在空间上分辨荧光团的浓度和寿命参数。然而，由于FT逆问题的不适定性，恢复的荧光团参数高度依赖于对象的大小和深度。来自另一种高空间分辨率成像方式的结构先验信息已被证明显著提高了FT重建的精度。在这项研究中，我们构建了一个磁共振成像(MRI)和FT相结合的小动物成像系统。采用光电倍增管(PMT)作为探测器，采集频域FT测量值。这是第一个与MR兼容的时间分辨FT系统，可以同时重建荧光浓度图和寿命图。通过模型研究对混合系统的性能进行了评估。利用了两种不同的荧光团：吲哚青绿(ICG)和3-3‘-二乙硫基三碳菁碘(DTTCI)，它们具有相似的激发和发射光谱，但寿命不同。为了比较，在有和没有结构先验信息的情况下，重建了荧光浓度图和寿命图。实验结果表明，当利用MRI结构先验信息时，该混合系统能够在10%的误差范围内准确地恢复直径为4.2 mm的两个圆柱体的荧光强度和寿命。
Fluorescence tomography (FT) is a promising molecular imaging technique that can spatially resolve both fluorophore concentration and lifetime parameters. However, recovered fluorophore parameters highly depend on the size and depth of the object due to the ill-posedness of the FT inverse problem. Structural a priori information from another high spatial resolution imaging modality has been demonstrated to significantly improve FT reconstruction accuracy. In this study, we have constructed a combined magnetic resonance imaging (MRI) and FT system for small animal imaging. A photo-multiplier tube (PMT) is used as the detector to acquire frequency domain FT measurements. This is the first MR-compatible time-resolved FT system that can reconstruct both fluorescence concentration and lifetime maps simultaneously. The performance of the hybrid system is evaluated with phantom studies. Two different fluorophores, Indocyanine Green (ICG) and 3-3′ Diethylthiatricarbocyanine Iodide (DTTCI), which have similar excitation and emission spectra but different lifetimes, are utilized. The fluorescence concentration and lifetime maps are both reconstructed with and without the structural a priori information obtained from MRI for comparison. We show that the hybrid system can accurately recover both fluorescence intensity and lifetime within 10% error for two 4.2 mm-diameter cylindrical objects embedded in a 38 mm-diameter cylindrical phantom when MRI structural a priori information is utilized.
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影响因子：
3.8
作者：
Lin Y;Barber WC;Iwanczyk JS;Roeck W;Nalcioglu O;Gulsen G
通讯作者：
Gulsen G
影响因子：
3.8
作者：
Ale, Angelique;Schulz, Ralf B.;Ntziachristos, Vasilis
通讯作者：
Ntziachristos, Vasilis
DOI：
10.1364/josaa.11.002727
发表时间：
1994-10-01
影响因子：
1.9
作者：
HASKELL, RC;SVAASAND, LO;TROMBERG, BJ
通讯作者：
TROMBERG, BJ
影响因子：
1.9
作者：
Milstein, AB;Oh, S;Millane, RP
通讯作者：
Millane, RP
DOI：
10.1016/j.jphotobiol.2009.11.007
发表时间：
2010-01-21
影响因子：
5.4
作者：
Leblond, Frederic;Davis, Scott C.;Valdes, Pablo A.;Pogue, Brian W.
通讯作者：
Pogue, Brian W.