Equivalency of the quality of sublethal lesions after photons and high-linear energy transfer ion beams.
Equivalency of the quality of sublethal lesions after photons and high-linear energy transfer ion beams.
复制标题
DOI：
10.1093/jrr/rrx030
复制
发表时间：
2017-11-01
影响因子：
2
通讯作者：
Konishi T
中科院分区：
文献类型：
--
作者：
Furusawa Y;Nakano-Aoki M;Matsumoto Y;Hirayama R;Kobayashi A;Konishi T
研究了高线性能量转移（LET）离子束辐照后的亚致死损伤（SLD）质量与低LET光子。在千叶工厂的重离子医用加速器中，首先将中国仓鼠V79细胞和人鳞状细胞癌SAS细胞暴露于不同LET的不同离子束的引发剂量。将细胞保持在室温下，然后暴露于第二测试剂量的X射线。基于修复动力学研究，细胞的存活分数随着修复时间的增加而迅速增加，并在2-3 h内达到平台，即使细胞接受预处理单能高LET光束或扩展布拉格峰光束以及X射线照射。在修复由高LET辐射引起的损伤后，来自二次测试X射线的细胞存活曲线的形状与仅暴露于初级X射线的细胞获得的形状相似。完整的SLD修复观察到，即使当初级离子束的LET是非常高的。这些结果表明，由高LET照射引起的SLD被很好地修复，同样，由X射线引起的损伤也被修复。在细胞中，离子束直接击中离子轨道的核心区域，对该区域产生致命损伤，导致细胞死亡。另一方面，在低LET半影区域中受到掠击的域中，产生的SLD被完全修复。
The quality of the sublethal damage (SLD) after irradiation with high–linear energy transfer (LET) ion beams was investigated with low-LET photons. Chinese hamster V79 cells and human squamous carcinoma SAS cells were first exposed to a priming dose of different ion beams at different LETs at the Heavy Ion Medical Accelerator in the Chiba facility. The cells were kept at room temperature and then exposed to a secondary test dose of X-rays. Based on the repair kinetics study, the surviving fraction of cells quickly increased with the repair time, and reached a plateau in 2–3 h, even when cells had received priming monoenergetic high-LET beams or spread-out Bragg peak beams as well as X-ray irradiation. The shapes of the cell survival curves from the secondary test X-rays, after repair of the damage caused by the high-LET irradiation, were similar to those obtained from cells exposed to primary X-rays only. Complete SLD repairs were observed, even when the LET of the primary ion beams was very high. These results suggest that the SLD caused by high-LET irradiation was repaired well, and likewise, the damage caused by the X-rays. In cells where the ion beam had made a direct hit in the core region in an ion track, lethal damage to the domain was produced, resulting in cell death. On the other hand, in domains that had received a glancing hit in the low-LET penumbra region, the SLD produced was completely repaired.
登录
查看更多内容
DOI：
10.1016/j.ijrobp.2015.11.034
发表时间：
2016-05-01
影响因子：
7
作者：
Karube, Masataka;Yamamoto, Naoyoshi;Kamada, Tadashi
通讯作者：
Kamada, Tadashi
影响因子：
3.4
作者：
Kase, Yuki;Kanai, Tatsuaki;Shinoda, Hiroshi
通讯作者：
Shinoda, Hiroshi
影响因子：
3.4
作者：
ELKIND, MM;SUTTONGI.H;SWAIN, RW
通讯作者：
SWAIN, RW
影响因子：
3.4
作者：
NGO, FQH;BLAKELY, EA;TOBIAS, CA
通讯作者：
TOBIAS, CA
影响因子：
2
作者：
Katz, R;Cucinotta, FA
通讯作者：
Cucinotta, FA