Optimization by Response Surface Methodology of Confluent and Aligned Cellular Monolayers for Nerve Guidance.
Optimization by Response Surface Methodology of Confluent and Aligned Cellular Monolayers for Nerve Guidance.
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DOI：
10.1007/s12195-009-0087-1
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发表时间：
2009-12
影响因子：
2.8
通讯作者：
Hoffman-Kim, Diane
中科院分区：
文献类型：
--
作者：
Kofron, Celinda M.;Hoffman-Kim, Diane
各向异性组织结构为体外和体内神经元的导航提供了指导。在这里，我们优化了星形胶质细胞、内皮细胞和雪旺细胞的可比较各向异性单层的生成，作为确定各向异性细胞的哪些特性足以用于神经引导的第一步。采用统计试验设计法和响应面法进行试验分析，提高效率和效用。研究的因素包括微接触印刷蛋白图案的尺寸、细胞密度和培养时间。蛋白质图案间距的影响最大。当细胞最初在边界排列并增殖以填充空间时，条纹之间的空间与细胞大小相当时是最有效的。最大化粘附分子覆盖面积对于这些类型细胞的融合也很重要。当细胞粘附并排列在条纹的宽度上并加宽以填充空间时，空间宽度约为细胞宽度的一半是最有效的。这些发现表明，如果排列机制，在边界或在条纹宽度上的排列，是预先确定的，并且细胞大小确定，则可以预测其他细胞类型的排列单层微图案的最佳尺寸。该研究还证明了DOE和RSM的有效使用，可以探测细胞对各种因素的反应，从而确定所需细胞反应的最佳条件。
Anisotropic tissue structures provide guidance for navigating neurons in vitro and in vivo. Here we optimized the generation of comparable anisotropic monolayers of astrocytes, endothelial cells, and Schwann cells as a first step toward determining which properties of anisotropic cells are sufficient for nerve guidance. The statistical experimental design method Design of Experiments and the experimental analysis method Response Surface Methodology were applied to improve efficiency and utility. Factors investigated included dimensions of microcontact printed protein patterns, cell density, and culture duration. Protein patterning spacing had the strongest influence. When cells initially aligned at borders and proliferated to fill in spaces, space between stripes was most effective when it was comparable to cell size. Maximizing the area of adhesive molecule coverage was also important for confluence of these types of cells. When cells adhered and aligned over the width of a stripe and broadened to fill spaces, space width about half the cell width was most effective. These findings suggest that if the mechanism of alignment, alignment at borders or over the width of the stripe, is predetermined and the cell size determined, the optimal size of the micropatterning for aligned monolayers of other cell types can be predicted. This study also demonstrates the effective use of DOE and RSM to probe cellular responses to various and multiple factors toward determination of optimal conditions for a desired cellular response.
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影响因子：
3
作者：
Li, Grace NgaYin;Hoffman-Kim, Diane
通讯作者：
Hoffman-Kim, Diane
DOI：
10.1007/bf01611732
发表时间：
1986-08-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
GLASBY, MA;GSCHMEISSNER, SG;HUANG, CLH
通讯作者：
HUANG, CLH
影响因子：
5.3
作者：
Dubey, N;Letourneau, PC;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
5.3
作者：
FoidartDessalle, M;Dubuisson, A;Lejeune, G
通讯作者：
Lejeune, G
DOI：
10.1590/s0100-879x2001000500008
发表时间：
2001-05-01
影响因子：
2.3
作者：
Gomes, FCA;Spohr, TCLS;Neto, VM
通讯作者：
Neto, VM