Direct and Functional Biomarkers of Vitamin B6 Status.
Direct and Functional Biomarkers of Vitamin B6 Status.
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DOI：
10.1146/annurev-nutr-071714-034330
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发表时间：
2015
影响因子：
8.9
通讯作者：
Gregory JF
中科院分区：
文献类型：
--
作者：
Ueland PM;Ulvik A;Rios-Avila L;Midttun Ø;Gregory JF
B6状态的测量分为直接生物标志物和功能生物标志物。直接生物标志物测量血浆/血清、尿液和红细胞中的B6维生素，其中血浆中最常用的是5-磷酸吡哆醛（PLP）。功能性生物标志物包括红细胞转氨酶活性和最近参与plp依赖反应的代谢物的血浆水平，如犬尿氨酸途径、单碳代谢、转硫（胱硫氨酸）和甘氨酸脱羧（丝氨酸和甘氨酸）。由于潜在的混杂因素，如炎症、碱性磷酸酶活性、低血清白蛋白、肾功能和无机磷酸盐的影响，维生素B6状态最好通过使用生物标志物的组合来评估。衬底-产物对之间的比率最近被研究作为一种策略，以减轻这种影响。这些工作为PAr指数、3-羟基尿氨酸/黄嘌呤酸比值和氧葡萄糖酸：谷氨酸比值等提供了有前景的新标记。基于质谱的靶向代谢分析或非靶向代谢组学允许同时定量大量代谢物，这些代谢物目前被评估为功能性生物标志物，使用数据还原统计。
Measures of B6 status are categorized as direct biomarkers and as functional biomarkers. Direct biomarkers measure B6 vitamers in plasma/serum, urine and erythrocytes, and among these plasma pyridoxal 5-phosphate (PLP) is most commonly used. Functional biomarkers include erythrocyte transaminase activities and more recently plasma levels of metabolites involved in PLP-dependent reactions, such as the kynurenine pathway, one-carbon metabolism, transsulfuration (cystathionine), and glycine decarboxylation (serine and glycine). Vitamin B6 status is best assessed by using a combination of biomarkers because of the influence of potential confounders, such as inflammation, alkaline phosphatase activity, low serum albumin, renal function and inorganic phosphate. Ratios between substrate-products pairs have recently been investigated as a strategy to attenuate such influence. These efforts have provided promising new markers such as the PAr index, the 3-hydroxykynurenine/xanthurenic acid ratio and the oxoglutarate:glutamate ratio. Targeted metabolic profiling or untargeted metabolomics based on mass spectrometry allow the simultaneous quantification of a large number of metabolites, which are currently evaluated as functional biomarkers, using data reduction statistics.
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影响因子：
37.8
作者：
Cattaneo, M;Lombardi, R;Mannucci, PM
通讯作者：
Mannucci, PM
影响因子：
8
作者：
Albersen, Monique;Groenendaal, Floris;Verhoeven-Duif, Nanda M.
通讯作者：
Verhoeven-Duif, Nanda M.
影响因子：
2.2
作者：
Apeland, T;Mansoor, MA;Strandjord, RE
通讯作者：
Strandjord, RE
影响因子：
3.6
作者：
Bates, CJ;Pentieva, KD;Finch, S
通讯作者：
Finch, S
影响因子：
7.1
作者：
ANDON, MB;REYNOLDS, RD;HOWARD, MP
通讯作者：
HOWARD, MP