Interaction of wild-type and mutant M protein vesicular stomatitis virus with nucleocapsids in vitro.
Interaction of wild-type and mutant M protein vesicular stomatitis virus with nucleocapsids in vitro.
复制标题
野生型和突变型 M 蛋白水泡性口炎病毒与核衣壳的体外相互作用。
DOI：
10.1021/bi00508a048
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Lenard,J
中科院分区：
文献类型：
--
作者：
Wilson,T;Lenard,J
材料和方法细胞和病毒。BHK 21-F细胞用于病毒原液的生长，Vero细胞用于所有噬斑试验。如前所述维持细胞（米勒& Lenard，1980）。VSV印第安纳州的奥赛（wtO）和格拉斯哥（wtG）变异体是从A. Huang，以及从A. Huang和A.弗兰芒通过以下程序分离这些突变体中的每一种的温度不敏感回复突变体。从在39 ℃下进行的III组突变体的测定中挑取单个噬斑，并重悬于Dulbecco磷酸盐缓冲盐水中。然后用这些悬浮液的稀释液（100倍）感染Vero细胞，在39 ℃孵育2天后，收集这些培养物的细胞上清液并在31和39 ℃下测定.如果培养物在两个温度下具有相当的滴度，则认为其是回复突变体，并从39 ℃测定中挑取噬斑。
Materials and MethodsCells and Virus. BHK 21-F cells were used for growth of virus stocks, and Vero cells were used for all plaque assays. Cells were maintained as described previously (Miller & Lenard, 1980). The orsay (wtO) and Glasgow (wtG) variants of VSV Indiana were obtained from A. Huang, and the group III mutants were obtained from A. Huang and A. Flamand. Temperature-insensitive revertants of each of these mutants were isolated by the following procedure. Individual plaques were picked from assays of group III mutants performed at 39 C and resuspended in Dulbecco’s phosphate-buffered saline. Dilutions (100-fold) of these suspensions were then used to infect Vero cells, and, following a 2-day incubation at 39 C, the cell supernatants of these cultures were collected and assayed at 31 and 39 C. If a culture had comparable titers at the two temperatures, it was considered to be a re-vertant, and plaques were picked from the 39 C assays.
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