IMPACT OF OSTEOCLAST PRECURSORS SUBJECTED TO RANDOM POSITIONING MACHINE ON OSTEOBLASTS
IMPACT OF OSTEOCLAST PRECURSORS SUBJECTED TO RANDOM POSITIONING MACHINE ON OSTEOBLASTS
复制标题
随机定位机对破骨细胞前体的影响对成骨细胞的影响
DOI：
10.1142/s0219519412005083
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发表时间：
2012-10
影响因子：
0.8
通讯作者：
Shang, Peng
中科院分区：
文献类型：
--
作者：
Di, Shengmeng;Meng, Rui;Qian, Airong;Tian, Zongcheng;Li, Jingbao;Zhang, Rong;Shang, Peng
成骨细胞-破骨细胞相互作用在骨重建中起着重要作用。在长时间的太空飞行中，宇航员会经历严重的骨丢失，这主要是由于骨形成和骨吸收之间的等效性被破坏。破骨细胞前体通常在成骨细胞的控制下运作。然而，在这里，我们表明，破骨细胞的前体可以反过来影响成骨细胞。RAW264.7细胞是小鼠破骨细胞的前体细胞，在随机定位机（RPM）产生的模拟失重条件下进行处理。72 h后，收集来自RPM（RCM）或静态对照（CCM）的RAW264.7细胞的条件培养基（CM），并用于培养成骨样MC 3 T3-E1细胞。结果表明，与CCM相比，RCM培养抑制了MC 3 T3-E1细胞的活力，并轻微改变了细胞周期，但未改变MC 3 T3-E1细胞的形态。此外，细胞内ALP水平，NO释放和成骨细胞标志基因的表达均被RCM培养下调。这些结果表明，破骨细胞前体细胞受到RPM施加成骨细胞的负调控。
Osteoblast-osteoclast interaction plays an important role in the bone remodeling. During long duration space flight, astronauts undergo serious bone loss mainly due to the disruption of equivalence between bone formation and bone resorption. Osteoclast precursors often operate under the control of osteoblasts. However, here we show that the osteoclast precursors could in turn influence osteoblasts. RAW264.7 cells, the murine osteoclast precursors, were treated in the simulated weightlessness produced by a Random Positioning Machine (RPM). After 72 h, conditioned mediums (CM) by the RAW264.7 cells from RPM (RCM) or static control (CCM) were collected and were used to culture osteoblastic-like MC3T3-E1 cells. The results showed that the RCM culture inhibited cell viability and slightly altered cell cycle, but the morphology of the MC3T3-E1 cells was not changed by RCM compared to that of CCM. Furthermore, the intracellular ALP level, NO release and expression of osteoblastic marker genes were all down-regulated by RCM culture. These results suggest that osteoclast precursors subjected to RPM exert negative regulation on osteoblasts.
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影响因子：
4.2
作者：
Dunlop, J. W. C.;Hartmann, M. A.;Brechet, Y. J.;Fratzl, P.;Weinkamer, R.
通讯作者：
Weinkamer, R.
影响因子：
3.7
作者：
HughesFulford, M;Lewis, ML
通讯作者：
Lewis, ML
影响因子：
3
作者：
L. Xiang;F. Qi;Dazhang Dai;Chun Xing Li;Y. Jiang
通讯作者：
L. Xiang;F. Qi;Dazhang Dai;Chun Xing Li;Y. Jiang
DOI：
--
发表时间：
1999-12
期刊：
Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University
影响因子：
--
作者：
D. Sarkar;T. Nagaya;K. Koga;H. Seo
通讯作者：
D. Sarkar;T. Nagaya;K. Koga;H. Seo
影响因子：
1.1
作者：
Doty, SB
通讯作者：
Doty, SB