Stored RBC metabolism as a function of caffeine levels.
Stored RBC metabolism as a function of caffeine levels.
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DOI：
10.1111/trf.15813
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发表时间：
2020-06
期刊：
影响因子：
2.9
通讯作者：
Recipient Epidemiology and Donor Evaluation Study-III (REDS III)
中科院分区：
文献类型：
--
作者：
D'Alessandro A;Fu X;Reisz JA;Kanias T;Page GP;Stone M;Kleinman S;Zimring JC;Busch M;Recipient Epidemiology and Donor Evaluation Study-III (REDS III)
咖啡消费在美国极为普遍。咖啡富含咖啡因，咖啡因是一种具有精神活性的腺苷受体嘌呤能拮抗剂，可调节红细胞能量和氧化还原代谢。由于红细胞（嘌呤）代谢是红细胞储存损伤的关键组成部分，因此我们着手研究咖啡因水平是否与储存红细胞中能量和氧化还原代谢的变化相关。我们通过超高压液相色谱与高分辨率质谱联用测量了 REDS-III RBC-Omics（受者流行病学捐赠者评估研究 III 红细胞组学）研究中的 599 个样本中的咖啡因及其主要代谢物的水平，并将它们与储存 10、23 和 42 天的红细胞的整体代谢组学和脂质组学分析相关联。咖啡因水平与主要红细胞抗氧化剂谷胱甘肽及其在氧化脂质和糖醛的谷胱甘肽依赖性解毒途径中的代谢中间体的水平增加呈正相关。咖啡因水平与转氨产物和底物、色氨酸和吲哚代谢物呈正相关。预计，由于咖啡因及其代谢物属于黄嘌呤嘌呤家族，因此咖啡因水平最高的受试者中所有黄嘌呤代谢物均显着增加。然而，尽管葡萄糖氧化产物通过糖酵解和磷酸戊糖途径减少，但高能磷酸盐化合物 ATP 和 DPG 并未受到咖啡因水平的影响。尽管是初步的，但这项研究表明咖啡因水平与储存红细胞抗氧化能力的提高之间存在有益的相关性。
Coffee consumption is extremely common in the United States. Coffee is rich with caffeine, a psychoactive, purinergic antagonist of adenosine receptors, which regulate red blood cell energy and redox metabolism. Since red blood cell (purine) metabolism is a critical component to the red cell storage lesion, here we set out to investigate whether caffeine levels correlated with alterations of energy and redox metabolism in stored red blood cells. We measured the levels of caffeine and its main metabolites in 599 samples from the REDS-III RBC-Omics (Recipient Epidemiology Donor Evaluation Study III Red Blood Cell-Omics) study via ultra-high-pressure-liquid chromatography coupled to high-resolution mass spectrometry and correlated them to global metabolomic and lipidomic analyses of RBCs stored for 10, 23, and 42 days. Caffeine levels positively correlated with increased levels of the main red cell antioxidant, glutathione, and its metabolic intermediates in glutathione-dependent detoxification pathways of oxidized lipids and sugar aldehydes. Caffeine levels were positively correlated with transamination products and substrates, tryptophan, and indole metabolites. Expectedly, since caffeine and its metabolites belong to the family of xanthine purines, all xanthine metabolites were significantly increased in the subjects with the highest levels of caffeine. However, high-energy phosphate compounds ATP and DPG were not affected by caffeine levels, despite decreases in glucose oxidation products—both via glycolysis and the pentose phosphate pathway. Though preliminary, this study is suggestive of a beneficial correlation between the caffeine levels and improved antioxidant capacity of stored red cells.
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影响因子：
1.3
作者：
Echeverri D;Montes FR;Cabrera M;Galán A;Prieto A
通讯作者：
Prieto A
影响因子：
2.9
作者：
Bertolone L;Roy MK;Hay AM;Morrison EJ;Stefanoni D;Fu X;Kanias T;Kleinman S;Dumont LJ;Stone M;Nemkov T;Busch MP;Zimring JC;D'Alessandro A
通讯作者：
D'Alessandro A
影响因子：
2.9
作者：
D'Alessandro A;Nemkov T;Kelher M;West FB;Schwindt RK;Banerjee A;Moore EE;Silliman CC;Hansen KC
通讯作者：
Hansen KC
影响因子：
7.1
作者：
DULLOO, AG;GEISSLER, CA;MILLER, DS
通讯作者：
MILLER, DS
影响因子：
2.9
作者：
DeSimone, Robert A.;Hayden, Joshua A.;Cushing, Melissa M.
通讯作者：
Cushing, Melissa M.