Neph/Nephrin-like adhesion and tissue level pulling forces regulate cell intercalation during Drosophila retina development
Neph/Nephrin-like adhesion and tissue level pulling forces regulate cell intercalation during Drosophila retina development
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Neph/Nephrin 样粘附和组织水平拉力调节果蝇视网膜发育过程中的细胞嵌入
DOI：
10.1101/564708
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Blackie L
中科院分区：
文献类型：
--
作者：
Blackie L
相邻细胞间的嵌入有助于上皮组织的形成，并受收缩肌动球蛋白细胞骨架的调节。虽然插层通常发生在几分钟内，但在器官发生过程中也报道了慢得多的细胞插层实例。例如，在果蝇视网膜形成过程中，可以观察到四个胶质样锥细胞（CC）。在这里，我们发现cc中的肌球蛋白ii活性在很大程度上是它们嵌入的必要条件。相反，我们发现CC四重奏中notch信号通路的差异活性调节嵌入，这也取决于细胞粘附蛋白Roughest和Hibris。此外，数学模型预测，插层CC四重奏外部的力是插层所必需的。与这一预测一致，我们发现周围的原代色素细胞处于显著的收缩张力之下。总之，我们的工作阐明了一种新的细胞嵌入模式，它依赖于Neph/ nephrin样粘附和嵌入细胞外部的力。
Intercalation between neighboring cells contributes to shaping epithelial tissues and is regulated by the contractile actomyosin cytoskeleton. While intercalation typically occurs over minutes, instances of much slower cell intercalation have been reported during organogenesis. This is observed, for example, for the four glial-like cone cells (CC) that intercalate duringDrosophilaretinal patterning. Here we show that Myosin-II activity in the CCs is largely dispensable for their intercalation. Instead, we find that differential activity of the Notch-signaling pathway within the CC quartet regulates intercalation, which also depends on the cell adhesion proteins Roughest and Hibris. In addition, mathematical modeling predicts that forces external to the intercalating CC quartet are necessary for intercalation. Consistent with this prediction we show that the surrounding primary pigment cells are under significant contractile tension. Altogether, our work elucidates a novel mode of cell intercalation that relies on Neph/Nephrin-like adhesion and forces external to the intercalating cells.
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影响因子：
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作者：
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通讯作者：
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