Contrast-enhanced imaging of SPIO-labeled platelets using magnetomotive ultrasound.
Contrast-enhanced imaging of SPIO-labeled platelets using magnetomotive ultrasound.
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DOI：
10.1088/0031-9155/58/20/7277
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发表时间：
2013-10-21
影响因子：
3.5
通讯作者：
Oldenburg AL
中科院分区：
文献类型：
--
作者：
Pope AG;Wu G;McWhorter FY;Merricks EP;Nichols TC;Czernuszewicz TJ;Gallippi CM;Oldenburg AL
在体内对血小板成像的能力可以提供对血液凝固过程和凝血病的洞察，并有助于识别与创伤或心血管疾病相关的血管内皮损伤部位。为此，我们开发了一种磁动超声（MMUS）系统，通过磁致振动提供超顺磁性氧化铁（SPIO）标记的血小板的对比度增强成像。血小板是一个有前途的平台，功能成像对比，因为他们很容易采取SPIO，很容易从血液中收获。在这里，我们报告了一种新的MMUS系统，可容纳任意厚的样品，同时保持便携性。我们采用了基于差分RF相位的带通滤波的频率锁定和相位锁定运动检测算法，该算法允许检测几纳米量级的亚分辨率振动幅度。然后，我们在SPIO浓度低至0.09 mg/ml Fe的同质组织模型中证明了MMUS（p < 0.0001，n = 6，t检验）。最后，我们证明了我们的系统能够对含有SPIO-血小板的185 μL模拟凝块进行三维成像。这突出了富血小板凝块的非侵入性成像的潜在效用，这将构成止血研究和临床相关血栓检测技术的根本进步。
The ability to image platelets in vivo can provide insight into blood clotting processes and coagulopathies, and aid in identifying sites of vascular endothelial damage related to trauma or cardiovascular disease. Toward this end, we have developed a magnetomotive ultrasound (MMUS) system that provides contrast-enhanced imaging of superparamagnetic iron oxide (SPIO) labeled platelets via magnetically-induced vibration. Platelets are a promising platform for functional imaging contrast because they readily take up SPIOs and are easily harvested from blood. Here we report a novel MMUS system that accommodates an arbitrarily thick sample while maintaining portability. We employed a frequency- and phase-locked motion detection algorithm based on bandpass filtering of the differential RF phase, which allows for the detection of sub-resolution vibration amplitudes on the order of several nanometers. We then demonstrated MMUS in homogenous tissue phantoms at SPIO concentrations as low as 0.09 mg/ml Fe (p < 0.0001, n = 6, t-test). Finally, we showed that our system is capable of 3-dimensional imaging of a 185 μL simulated clot containing SPIO-platelets. This highlights the potential utility for non-invasive imaging of platelet-rich clots, which would constitute a fundamental advance in technology for the study of hemostasis and detection of clinically relevant thrombi.
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影响因子：
3.8
作者：
Oldenburg, AL;Toublan, FJJ;Boppart, SA
通讯作者：
Boppart, SA
影响因子：
3.8
作者：
Oldenburg, Amy L.;Crecea, Vasilica;Boppart, Stephen A.
通讯作者：
Boppart, Stephen A.
影响因子：
2.9
作者：
Piedra, Mark;Allroggen, Achim;Lindner, Jonathan R.
通讯作者：
Lindner, Jonathan R.
影响因子：
2.8
作者：
Patil AV;Rychak JJ;Klibanov AL;Hossack JA
通讯作者：
Hossack JA
影响因子：
82.9
作者：
Falati, S;Gross, P;Furie, B
通讯作者：
Furie, B