Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in deer in Henan and Jilin, China.
Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in deer in Henan and Jilin, China.
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隐孢子虫属的流行率和分子特征。
DOI：
10.1186/s13071-018-2813-9
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发表时间：
2018-04-12
影响因子：
3.2
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Huang J;Zhang Z;Zhang Y;Yang Y;Zhao J;Wang R;Jian F;Ning C;Zhang W;Zhang L
人们对隐孢子虫属的流行率和人畜共患潜力知之甚少。以及中国鹿体内的十二指肠贾第鞭毛虫。本研究于2013年7月至2014年8月期间从河南省和吉林省的11个养殖场采集了662份粪便样本，并通过基因分型和亚分型方法筛查了隐孢子虫和十二指肠球菌的存在。隐孢子虫属和 G. duodenalis 分别在 6.80% (45/662) 和 1.21% (5/662) 的样本中检出。根据小亚基核糖体核糖核酸 (SSU rRNA) 基因鉴定了六种隐孢子虫物种/基因型：C. parvum (n = 11)； C. 安德森 (n = 5)； C. 无处不在（n = 3）； C. muris (n = 1); C. 类猪 (n = 1)；和隐孢子虫鹿基因型 (n = 24)。通过对 60 kDa 糖蛋白 (gp60) 基因进行测序，对 11 个微小隐球菌分离株中的 5 个进行亚型分型，发现人畜共患亚型 IIaA15G2R2 (n = 4) 和 IIdA19G1 (n = 1)。根据亚型分析，3个泛素棒杆菌属于XIIa亚型2。相比之下，通过小亚基核糖体核糖核酸（SSU rRNA）基因测序，在5个贾第鞭毛虫阳性样本中仅检测到组合E。据我们所知，这是第一项报告鹿科动物中的 C. andersoni 以及 C. parvum 人畜共患亚型 IIaA15G2R2 和 IIdA19G1 的研究。这些数据虽然有限，但表明鹿科动物可能是人畜共患的隐孢子虫和贾第鞭毛虫的来源。本研究中的鹿科动物对人类的人畜共患病可能性较低，需要更多的分子流行病学研究来阐明隐孢子虫和十二指肠球菌在中国各地鹿科动物中的流行情况和公共卫生意义。
Little is known about the prevalence and zoonotic potential of Cryptosporidium spp. and Giardia duodenalis in deer in China. In this study, 662 fecal samples were collected from 11 farms in Henan and Jilin Provinces between July 2013 and August 2014, and were screened for the presence of Cryptosporidium and G. duodenalis with genotyping and subtyping methods. Cryptosporidium spp. and G. duodenalis were detected in 6.80% (45/662) and 1.21% (5/662) of samples, respectively. Six Cryptosporidium species/genotypes were identified based on the small subunit ribosomal ribonucleic acid (SSU rRNA) gene: C. parvum (n = 11); C. andersoni (n = 5); C. ubiquitum (n = 3); C. muris (n = 1); C. suis-like (n = 1); and Cryptosporidium deer genotype (n = 24). When five of the 11 C. parvum isolates were subtyped by sequencing the 60 kDa glycoprotein (gp60) gene, zoonotic subtypes IIaA15G2R2 (n = 4) and IIdA19G1 (n = 1) were found. According to a subtype analysis, three C. ubiquitum isolates belonged to XIIa subtype 2. In contrast, only assemblage E was detected in the five Giardia-positive samples with small subunit ribosomal ribonucleic acid (SSU rRNA) gene sequencing. To our knowledge, this is the first study to report C. andersoni, as well as C. parvum zoonotic subtypes IIaA15G2R2 and IIdA19G1 in cervids. These data, though limited, suggest that cervids may be a source of zoonotic Cryptosporidium and Giardia. Cervids in the present study are likely to be of low zoonotic potential to humans, and more molecular epidemiological studies are required to clarify the prevalence and public health significance of Cryptosporidium and G. duodenalis in cervids throughout China.
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影响因子：
2.1
作者：
Beck, Relja;Sprong, Hein;Caccio, Simone M.
通讯作者：
Caccio, Simone M.
影响因子：
6.4
作者：
Fantinatti, Maria;Bello, Alexandre R.;Da-Cruz, Alda M.
通讯作者：
Da-Cruz, Alda M.
影响因子：
2.6
作者：
Hamnes, Inger Sofie;Gjerde, Bjorn;Handeland, Kjell
通讯作者：
Handeland, Kjell
影响因子：
2.6
作者：
Amer, Said;Honma, Hajime;Nakai, Yutaka
通讯作者：
Nakai, Yutaka
影响因子：
2
作者：
Foronda, P.;Bargues, M. D.;Mas-Coma, S.
通讯作者：
Mas-Coma, S.