Proteomic analysis of early response lymph node proteins in mice treated with titanium dioxide nanoparticles.
Proteomic analysis of early response lymph node proteins in mice treated with titanium dioxide nanoparticles.
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DOI：
10.1016/j.jprot.2011.08.009
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发表时间：
2011-11-18
影响因子：
3.3
通讯作者：
Yu LR
中科院分区：
文献类型：
--
作者：
Gao Y;Gopee NV;Howard PC;Yu LR
人类暴露于纳米粒子是不可避免的自然和人为来源。二氧化钛（TiO 2）纳米颗粒越来越多地用于制药和化妆品。先前的研究表明，组织中的TiO 2水平显著增加（例如，淋巴结）。为了鉴定对TiO 2纳米颗粒的早期反应淋巴结蛋白，用低剂量的DeGussa P25 TiO 2纳米颗粒或单独的载体皮内注射小鼠组。采用胰蛋白酶催化的16 O/18 O标记结合二维液相色谱-串联质谱（2DLC-MS/MS）对24 h淋巴结蛋白质组进行定量分析。在用TiO 2纳米颗粒处理的小鼠中，总共有33种蛋白质发生显著变化（超过1.3倍，p<0.05），约占鉴定的总蛋白质的1%。差异表达的蛋白质主要涉及免疫应答（例如，炎症）、脂质和脂肪酸代谢、mRNA加工和核小体组装。雌激素受体（ESR 1）、过氧化物酶体增殖物激活受体γ（PPARγ）和c-Myc信号通路等可介导不同功能蛋白的调节。本实验中鉴定的差异表达蛋白可代表小鼠淋巴结对纳米TiO 2处理的早期反应蛋白。
Human exposure to nanoparticles is inevitable from natural and anthropogenic sources. Titanium dioxide (TiO2) nanoparticles are increasingly being used in pharmaceutical and cosmetic products. Previous studies revealed that TiO2 levels were significantly increased in tissues (e.g., lymph nodes) after mice were injected with nanosized TiO2. To identify early response lymph node proteins to TiO2 nanoparticles, groups of mice were intradermally injected with a low dose of DeGussa P25 TiO2 nanoparticles or vehicle alone. The proteomes of lymph nodes at 24 h were quantitatively analyzed using trypsin-catalyzed 16O/18O labeling in conjunction with two-dimensional liquid chromatography separation and tandem mass spectrometry (2DLC-MS/MS). A total of 33 proteins were significantly changed (over 1.3-fold, p<0.05) in the mice treated with TiO2 nanoparticles, which accounted for approximately 1% of the total proteins identified. The differentially expressed proteins mainly involve the immune response (e.g., inflammation), lipid and fatty acid metabolism, mRNA processing, and nucleosome assembly. Regulation of functionally distinct classes of proteins could be mediated by estrogen receptor (ESR1), PPARγ, and c-Myc signalings, etc. The differentially expressed proteins identified in this experiment could represent early response proteins to TiO2 nanoparticle treatment in mouse lymph nodes.
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影响因子：
30.8
作者：
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通讯作者：
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影响因子：
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DOI：
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