Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.
Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.
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DOI：
10.1016/0042-6822(91)90760-9
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发表时间：
1991-11
期刊：
影响因子：
3.7
通讯作者：
Reyes GR
中科院分区：
文献类型：
--
作者：
Tam AW;Smith MM;Guerra ME;Huang CC;Bradley DW;Fry KE;Reyes GR
我们最近描述了部分戊型肝炎病毒 (HEV) 的克隆，并证实了其与肠道传播（水源性、流行性）非甲型、非乙型肝炎的病因学关联。该病毒由约 7.5 kb 的单链正义 RNA 基因组组成，具有聚腺苷酸化 3' 末端。我们现在报告了一组重叠、连续的 cDNA 克隆的克隆和核苷酸测序，这些克隆代表了 HEV 缅甸毒株 [HEV(B)] 的整个基因组。最大的开放阅读框从 Fend 延伸约 5 kb，包含 RNA 指导的 RNA 聚合酶和三磷酸核苷结合基序。第二个主要开放阅读框 (ORF2) 开始于第一个主要开放阅读框的下游 37 by，并从 Poly(A) 残基的 3' 末端延伸段延伸约 2 kb 至 65 by 处的终止密码子。 ORF2 在其氨基末端包含共有信号肽序列和衣壳样区域，该区域具有高含量的碱性氨基酸，与其他病毒衣壳蛋白相似。第三个开放阅读框与第一个和第二个开放阅读框部分重叠，仅包含 369 bp。除了 7.5 kb 全长基因组转录本外，使用来自基因组 3' 三分之一的探针在受感染的肝脏中检测到了两个大约 3.7 和 2.0 kb 的亚基因组聚腺苷酸化信息。病毒的基因组组织与编码非结构基因的Fend和编码病毒结构基因的3'端一致。病毒的表达策略涉及使用三种不同的开放阅读框和至少三种不同的转录本。 HEV 先前被确定为直径为 27-34 nm 的无包膜颗粒。这些关于 HEV 遗传组织和表达策略的发现表明，它是一类新型 RNA 病毒或可能是杯状病毒科中的一个单独属的人类病原体原型
We have recently described the cloning of a portion of the hepatitis E virus (HEV) and confirmed its etiologic association with enterically transmitted (waterborne, epidemic) non-A, non-B hepatitis. The virus consists of a single-stranded, positive-sense RNA genome of approximately 7.5 kb, with a polyadenylated 3' end. We now report on the cloning and nucleotide sequencing of an overlapping, contiguous set of cDNA clones representing the entire genome of the HEV Burma strain [HEV(B)]. The largest open reading frame extends approximately 5 kb from the Fend and contains the RNA-directed RNA polymerase and nucleoside triphosphate binding motifs. The second major open reading frame (ORF2) begins 37 by downstream of the first and extends approximately 2 kb to the termination codon present 65 by from the 3' terminal stretch of poly(A) residues. ORF2 contains a consensus signal peptide sequence at its amino terminus and a capsid-like region with a high content of basic amino acids similar to that seen with other virus capsid proteins. A third open reading frame partially overlaps the first and second and encompasses only 369 bp. In addition to the 7.5-kb full-length genomic transcript, two subgenomic polyadenylated messages of approximately 3.7 and 2.0 kb were detected in infected liver using a probe from the 3' third of the genome. The genomic organization of the virus is consistent with the Fend encoding nonstructural and the 3' end encoding the viral structural gene(s). The expression strategy of the virus involves the use of three different open reading frames and at least three different transcripts. HEV was previously determined to be a nonenveloped particle with a diameter of 27–34 nm. These findings on the genetic organization and expression strategy of HEV suggest that it is the prototype human pathogen for a new class of RNA virus or perhaps a separate genus within the Caliciviridae family
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影响因子：
14.9
作者：
Gorbalenya AE;Koonin EV;Donchenko AP;Blinov VM
通讯作者：
Blinov VM
影响因子：
14.9
作者：
KUBO, Y;TAKEUCHI, K;MIYAMURA, T
通讯作者：
MIYAMURA, T
影响因子：
56.9
作者：
KUO, G;CHOO, QL;HOUGHTON, M
通讯作者：
HOUGHTON, M
影响因子：
14.9
作者：
MAENO, M;KAMINAKA, K;SHIKATA, T
通讯作者：
SHIKATA, T
影响因子：
158.5
作者：
FEINSTONE, SM;KAPIKIAN, AZ;HOLLAND, PV
通讯作者：
HOLLAND, PV