Synchronized Stepped Frequency Modulation for Multiplexed Ion Mobility Measurements.
Synchronized Stepped Frequency Modulation for Multiplexed Ion Mobility Measurements.
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DOI：
10.1021/jasms.1c00365
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发表时间：
2022-03-02
影响因子：
3.2
通讯作者：
Clowers, Brian H.
中科院分区：
文献类型：
--
作者：
Cabrera, Elvin R.;Clowers, Brian H.
用于离子迁移谱（例如，傅里叶变换离子迁移谱，（FT-IMS））的频率编码多路复用的实现已经促进了漂移管离子迁移率仪器与离子阱质量分析器的直接耦合，尽管它们的占空比失配。传统上，FT-IMS实验已经利用独立于离子阱质量分析器的扫描速率的连续线性频率扫描来进行;因此，产生了在两个连续质量扫描上扫描多个频率的情况。这又产生了一定程度的模糊性，其中从单个调制频率导出的离子电流不能被分配给频率调制信号中的单个数据点。为了消除这种模糊性，这项工作描述了一个离散的逐步函数来调制IMS的离子门，同时实现所产生的频率和线性离子阱的扫描速率之间的同步。虽然步进频率扫描中使用的单个频率的数量少于连续线性调制实验中使用的单个频率的数量，但是在终端频率和扫描长度的不同组合上没有性能损失并且保持高水平的精度。此外，频率扫描同步使得进一步的数据处理技术（例如，频率调制信号的线性平均）能够显著提高高强度和低强度分析物的信噪比。
Implementation of frequency encoded multiplexing for ion mobility spectrometry (e.g. Fourier Transform Ion Mobility Spectrometry, (FT-IMS)) has facilitated the direct coupling of drift tube ion mobility instrumentation with ion trap mass analyzers despite their duty cycle mismatch. Traditionally, FT-IMS experiments have been carried out utilizing continuous linear frequency sweeps that are independent of the scan rate of the ion trap mass analyzer; thus, creating a situation where multiple frequencies are swept over two sequential mass scans. This in turn creates a degree of ambiguity in which the ion current derived from a single modulation frequency cannot be assigned to a single datapoint in the frequency modulated signal. In an effort to eliminate this ambiguity, this work describes a discrete stepwise function to modulate the ion gates of the IMS while synchronization between the generated frequencies and the scan rate of the linear ion trap is achieved. While the number of individual frequencies used in the stepped frequency sweeps is less than in continuous linear modulation experiments, there is no loss in performance and maintain high levels of precision across differing combinations of terminal frequencies and scan lengths. Furthermore, the frequency-scan synchronization enables further data processing techniques such as linear averaging of the frequency modulated signal to drastically improve signal-to-noise ratio for both high and low intensity analytes.
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影响因子：
7.4
作者：
McCabe JW;Shirzadeh M;Walker TE;Lin CW;Jones BJ;Wysocki VH;Barondeau DP;Clemmer DE;Laganowsky A;Russell DH
通讯作者：
Russell DH
影响因子：
7.4
作者：
Poltash ML;McCabe JW;Shirzadeh M;Laganowsky A;Clowers BH;Russell DH
通讯作者：
Russell DH
DOI：
10.1007/s13361-019-02288-2
发表时间：
2019-11-01
影响因子：
3.2
作者：
Dodds, James N.;Baker, Erin S.
通讯作者：
Baker, Erin S.
影响因子：
7.4
作者：
KNORR, FJ;EATHERTON, RL;HILL, HH
通讯作者：
HILL, HH
DOI：
10.1021/jasms.1c00184
发表时间：
2021-08-05
影响因子：
3.2
作者：
McKenna, Kristin R.;Clowers, Brian H.;Fernandez, Facundo M.
通讯作者：
Fernandez, Facundo M.