Physiologically evoked neuronal current MRI in a bloodless turtle brain: detectable or not?
Physiologically evoked neuronal current MRI in a bloodless turtle brain: detectable or not?
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DOI：
10.1016/j.neuroimage.2009.06.017
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发表时间：
2009-10-01
期刊：
影响因子：
5.7
通讯作者：
Gao, Jia-Hong
中科院分区：
文献类型：
--
作者：
Luo, Qingfei;Lu, Huo;Lu, Hanbing;Senseman, David;Worsley, Keith;Yang, Yihong;Gao, Jia-Hong
从检测诱发神经元电流的努力矛盾的结果已经离开了神经元电流MRI（ncMRI）的可行性一个悬而未决的问题。由于无法分离或消除血流动力学影响，大多数先前在人类受试者中进行的ncMRI研究都是可疑的。在本研究中，我们使用了一个无血的龟脑，消除了血液动力学的影响，探讨在9.4T的视觉诱发ncMRI信号检测的可行性。海龟的大脑，连同它的眼睛，被从头盖骨上解剖下来，放在人工脑脊液中。闪光被传递到眼睛，在大脑中产生视觉诱发的神经元活动。局部场电位（LFP）和磁共振成像信号在龟脑中的交错方式进行了测量。虽然在LFP信号中观察到对视觉刺激的强烈神经元反应，但在MRI图像中没有发现与神经元电流同步的显著信号变化。MRI时间过程的时间稳定性分析表明，幅度和相位的可检测效应大小分别为0.11%和0.09°，龟脑中视觉诱发的ncMRI信号低于这些水平。
Contradictory results from the efforts for detecting evoked neuronal currents have left the feasibility of neuronal current MRI (ncMRI) an open question. Most of the previous ncMRI studies in human subjects are suspect due to their inability to separate or eliminate the hemodynamic effects. In this study, we used a bloodless turtle brain that eliminates hemodynamic effects, to explore the feasibility of detecting visual-evoked ncMRI signals at 9.4T. The turtle brain, with its eyes attached, was dissected from the cranium and placed in artificial cerebral spinal fluid. Light flashes were delivered to the eyes, which produced visual-evoked neuronal activity in the brain. Local field potential (LFP) and MRI signals in the turtle brain were measured in an interleave fashion. Although robust neuronal responses to the visual stimulation were observed in the LFP signals, no significant signal changes synchronized with neuronal currents were found in the MRI images. Analysis of the temporal stability of the MRI time courses indicated that the detectable effect sizes are 0.11% and 0.09° for the magnitude and phase, respectively, and the visual-evoked ncMRI signals in the turtle brain are below these levels.
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