Changes in surface morphology of Chinese hamster ovary cells during the cell cycle.
Changes in surface morphology of Chinese hamster ovary cells during the cell cycle.
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DOI：
10.1083/jcb.57.3.815
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发表时间：
1973-06
期刊：
影响因子：
--
通讯作者：
Frye J
中科院分区：
文献类型：
--
作者：
Porter K;Prescott D;Frye J
中国仓鼠卵巢（CHO）细胞在汇合培养的同步人口已被检查，通过扫描电子显微镜和他们的表面变化，通过周期的细胞进展。在G1期，细胞的特征是显示大量的微绒毛、大泡和皱褶。除皱褶外，在S期，这些皱褶的突出程度趋于减少，细胞变得相对光滑，因为它们在基质上稀疏地分布。在G2期，微绒毛数量增加，细胞聚集，预期进行有丝分裂。这里观察到的变化似乎反映了CHO细胞在周期中对与群体中其他细胞接触的反应能力的变化，因为正如随后的论文（Rubin和Everhart）所指出的，在稀疏的非融合培养物中的CHO细胞在细胞周期中没有显示出同样广泛的变化。在融合培养中，相同类型的正常非转化细胞基本上没有微绒毛、大泡和皱褶。这些表面配置的细胞的内部结构的关系进行了讨论。
Synchronized populations of Chinese hamster ovary (CHO) cells in confluent culture have been examined by scanning electron microscopy and their surface changes noted as the cells progress through the cycle. During G1 it is characteristic for cells to show large numbers of microvilli, blebs, and ruffles. Except for the ruffles, these tend to diminish in prominence during S and the cells become relatively smooth as they spread thinly over the substrate. During G2 microvilli increase in number and the cells thicken in anticipation of rounding up for mitosis. It appears that the changes observed here reflect the changing capacity of CHO cells during the cycle to respond to contact with other cells in the population, because, as noted in the succeeding paper (Rubin and Everhart), CHO cells in sparse nonconfluent cultures do not show the same wide range of changes during the cell cycle. Normal, nontransformed cells of equivalent type in confluent culture are essentially devoid of microvilli, blebs, and ruffles. The relation of these surface configurations to the internal structure of the cell is discussed.
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影响因子：
64.8
作者：
INGRAM, VM
通讯作者：
INGRAM, VM
影响因子：
56.9
作者：
JOHNSON, RG;SHERIAN, JD
通讯作者：
SHERIAN, JD
影响因子：
3.7
作者：
ABERCROM.M;HEAYSMAN, JE;PEGRUM, SM
通讯作者：
PEGRUM, SM
DOI：
10.1073/pnas.68.7.1648
发表时间：
1971-01-01
影响因子：
11.1
作者：
HSIE, AW;JONES, C;PUCK, TT
通讯作者：
PUCK, TT
影响因子：
3.7
作者：
BOSTOCK, CJ;PRESCOTT, DM;KIRKPATRICK, JB
通讯作者：
KIRKPATRICK, JB