Molecular analysis of neocortical layer structure in the ferret.
Molecular analysis of neocortical layer structure in the ferret.
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DOI：
10.1002/cne.22399
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发表时间：
2010-08-15
期刊：
影响因子：
--
通讯作者：
Ragsdale CW
中科院分区：
文献类型：
--
作者：
Rowell JJ;Mallik AK;Dugas-Ford J;Ragsdale CW
区分啮齿动物新皮层特定层的分子标记越来越多地用于研究皮层发育和皮层回路的生理学。然而，这些标记物在多大程度上代表了哺乳动物新皮层细胞类型身份的一般特征尚不清楚。为了更广泛地评估层标记的保守性，我们分离了雪貂（一种具有大型脑回的食肉动物）中15个层富集基因的直系同源物，并分析了它们的新皮质基因表达模式。我们的主要发现是：（1）许多但不是所有测试的层标记显示小鼠和雪貂皮层之间层特异性基因表达的相似模式，支持层特异性细胞类型同一性在哺乳动物总目中在分子水平上保守的观点;（2）我们的深层标记面板（ER81/ETV 1，SULF 2，PCP 4，FEZF 2/ZNF 312，CACNA 1H，KCNN 2/SK 2，SYT 6，FOXP 2，CTGF）提供了分子证据，表明第5层和第6层的特定分层为5a，5 b，6a和6 b在啮齿动物和食肉动物之间也是保守的。(3)雪貂皮层各区域的层特异性基因表达的变化比小鼠和雪貂皮层同源区域之间的变化更明显;（4）区域基因表达的这种变化在所研究的浅层标记物（SERPINE 2、MDGA 1、CUX 1、UNC 5D、RORB/NR 1F 2、EAG 2/KCNH 5）中最明显。最引人注目的是，第4层标记RORB和EAG 2揭示了雪貂视觉皮层的分子亚层，并证明了所谓的新皮层无颗粒区域之间的分子解离。我们的研究结果确立了分子标记物作为细胞结构的有力补充，用于哺乳动物的新皮质层和细胞类型比较。
Molecular markers that distinguish specific layers of rodent neocortex are increasingly employed to study cortical development and the physiology of cortical circuits. The extent to which these markers represent general features of neocortical cell type identity across mammals is, however, unknown. To assess the conservation of layer markers more broadly, we isolated orthologs for fifteen layer-enriched genes in the ferret, a carnivore with a large, gyrencephalic brain, and analyzed their patterns of neocortical gene expression. Our major findings are: (1) Many but not all layer markers tested show similar patterns of layer-specific gene expression between mouse and ferret cortex, supporting the view that layer-specific cell type identity is conserved at a molecular level across mammalian superorders; (2) Our panel of deep layer markers (ER81/ETV1, SULF2, PCP4, FEZF2/ZNF312, CACNA1H, KCNN2/SK2, SYT6, FOXP2, CTGF) provides molecular evidence that the specific stratifications of layer 5 and 6 into 5a, 5b, 6a and 6b are also conserved between rodents and carnivores. (3) Variations in layer-specific gene expression are more pronounced across areas of ferret cortex than between homologous areas of mouse and ferret cortex; (4) This variation of area gene expression was clearest with the superficial layer markers studied (SERPINE2, MDGA1, CUX1, UNC5D, RORB/NR1F2, EAG2/KCNH5). Most dramatically, the layer 4 markers RORB and EAG2 disclosed a molecular sublamination to ferret visual cortex and demonstrated a molecular dissociation among the so-called agranular areas of the neocortex. Our findings establish molecular markers as a powerful complement to cytoarchitecture for neocortical layer and cell-type comparisons across mammals.
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影响因子：
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