Pharmacokinetics, Biodistribution, and Biosafety of PEGylated Gold Nanoparticles In Vivo.
Pharmacokinetics, Biodistribution, and Biosafety of PEGylated Gold Nanoparticles In Vivo.
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DOI：
10.3390/nano11071702
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发表时间：
2021-06-28
期刊：
影响因子：
--
通讯作者：
Gabelova A
中科院分区：
文献类型：
--
作者：
Kozics K;Sramkova M;Kopecka K;Begerova P;Manova A;Krivosikova Z;Sevcikova Z;Liskova A;Rollerova E;Dubaj T;Puntes V;Wsolova L;Simon P;Tulinska J;Gabelova A
尽管金纳米粒子在生物医学应用中具有明显的优势，但有争议的和不完整的毒理学数据阻碍了它们的广泛使用。在这里，我们介绍了使用聚乙二醇包覆的金纳米颗粒(PEG-AuNPs)进行的体内毒性研究的结果。单次静脉注射后，研究了聚乙二醇金纳米粒在大鼠肝、肺、脾和肾中的药代动力学和生物分布。不同时间间隔注射(0.7 mg/kg)。聚乙二醇金纳米粒具有相对较长的血液循环时间，主要聚集在肝脏和脾中，在给药后保留长达28天。染毒后24小时和7天，肝细胞胞浆空泡化增加，染毒后24小时白脾髓内可见凋亡样细胞，而染毒28天后未见明显的细胞凋亡。相反，在这个时间点，我们发现脂代谢发生了显著变化，肝损伤标志物水平改变，单核细胞计数增加，但没有明显的生物学相关性。在血细胞中，除了在注射4h后在原代肾细胞中检测到短暂的DNA断裂外，在任何研究的时间间隔内都没有出现DNA损伤。我们的结果表明，聚乙二醇金纳米粒在组织中的蓄积可能导致晚期毒性效应。
Despite the obvious advantages of gold nanoparticles for biomedical applications, controversial and incomplete toxicological data hamper their widespread use. Here, we present the results from an in vivo toxicity study using gold nanoparticles coated with polyethylene glycol (PEG-AuNPs). The pharmacokinetics and biodistribution of PEG-AuNPs were examined in the rat’s liver, lung, spleen, and kidney after a single i.v. injection (0.7 mg/kg) at different time intervals. PEG-AuNPs had a relatively long blood circulation time and accumulated primarily in the liver and spleen, where they remained for up to 28 days after administration. Increased cytoplasmic vacuolation in hepatocytes 24 h and 7 days after PEG-AuNPs exposure and apoptotic-like cells in white splenic pulp 24 h after administration has been detected, however, 28 days post-exposure were no longer observed. In contrast, at this time point, we identified significant changes in lipid metabolism, altered levels of liver injury markers, and elevated monocyte count, but without marked biological relevance. In blood cells, no DNA damage was present in any of the studied time intervals, with the exception of DNA breakage transiently detected in primary kidney cells 4 h post-injection. Our results indicate that the tissue accumulation of PEG-AuNPs might result in late toxic effects.
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DOI：
10.1016/j.nano.2014.06.005
发表时间：
2014-11-01
影响因子：
5.4
作者：
Fraga, Sonia;Brandao, Ana;Carmo, Helena
通讯作者：
Carmo, Helena
影响因子：
10
作者：
Chou, Leo Y. T.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
4.4
作者：
Bednarski, Marek;Dudek, Magdalena;Tesiorowski, Maciej
通讯作者：
Tesiorowski, Maciej
影响因子：
4.4
作者：
Abdelhalim, Mohamed Anwar K.;Moussa, Sherif A. Abdelmottaleb
通讯作者：
Moussa, Sherif A. Abdelmottaleb
DOI：
10.3390/nano5020835
发表时间：
2015-05-21
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Jo MR;Bae SH;Go MR;Kim HJ;Hwang YG;Choi SJ
通讯作者：
Choi SJ