Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women.
Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women.
复制标题
DOI：
10.1186/s12903-022-02293-0
复制
发表时间：
2022-06-28
期刊：
影响因子：
2.9
通讯作者：
Furuichi, Yasushi
中科院分区：
文献类型：
--
作者：
Kato, Satsuki;Nagasawa, Toshiyuki;Uehara, Osamu;Shimizu, Shintaro;Sugiyama, Nodoka;Hasegawa-Nakamura, Kozue;Noguchi, Kazuyuki;Hatae, Masayuki;Kakinoki, Hiroshige;Furuichi, Yasushi
孕妇体内共生菌群的建立对母亲及其后代都很重要。人们对妊娠期唾液共生菌知之甚少，微生物组组成分析（ANCOM）可用于检测细菌数量的微小差异。本研究的目的是利用ANCOM来研究健康孕妇和非孕妇唾液细菌的差异。从35名妊娠35周的健康孕妇和30名月经期间的健康非孕妇收集未刺激的唾液样品。所有参与者都接受了牙周检查。采用酶联免疫吸附试验检测血清雌二醇和孕酮水平。从唾液中提取的DNA通过16 S核糖体RNA扩增子测序和实时PCR进行评估。孕妇唾液雌二醇和孕酮水平显著升高。孕妇的α和β-维生素D水平高于非孕妇。当分析孕妇和非孕妇的微生物群时，注意到的最大效应量差异是双歧杆菌。在孕妇中，牙双歧杆菌的水平显著升高，而双歧杆菌的水平没有显著升高，并且该水平与孕酮浓度显著相关。结果表明，健康孕妇唾液中双歧杆菌和孕酮水平高于非孕妇。
The establishment of symbiotic microbiota in pregnant women is important for both the mother and her offspring. Little is known about the salivary symbiotic bacteria in pregnancy, and analysis of composition of microbiome (ANCOM) is useful to detect small differences in the number of bacteria. The aim of this study was to investigate the differences in the salivary bacteria between healthy pregnant and non-pregnant women using ANCOM. Unstimulated saliva samples were collected from 35 healthy pregnant women at 35 weeks gestation and 30 healthy non-pregnant women during menstruation. All participants underwent a periodontal examination. Estradiol and progesterone levels were examined by enzyme-linked immunosorbent assay. DNA extracted from the saliva was assessed by 16S ribosomal RNA amplicon sequencing and real-time PCR. Salivary estradiol and progesterone levels were significantly increased in pregnant women. The alpha and beta diversities were higher in pregnant women than in non-pregnant women. The largest effect size difference noted when the microbiota of the pregnant and non-pregnant women were analyzed was that for Bifidobacteriales. Levels of Bifidobacterium dentium, but not of Bifidobacterium adolescentis, were significantly increased in pregnant women, and the levels were significantly correlated with progesterone concentration. The results suggest that Bifidobacterium and progesterone levels are elevated in the saliva of healthy pregnant women compared with non-pregnant women.
登录
查看更多内容
影响因子：
--
作者：
Daalderop LA;Wieland BV;Tomsin K;Reyes L;Kramer BW;Vanterpool SF;Been JV
通讯作者：
Been JV
影响因子：
6.7
作者：
Carrillo-de-Albornoz, Ana;Figuero, Elena;Bascones-Martinez, Antonio
通讯作者：
Bascones-Martinez, Antonio
影响因子：
4.5
作者：
Ishikawa, Karin Hitomi;Mita, Daniela;Alves Mayer, Marcia Pinto
通讯作者：
Alves Mayer, Marcia Pinto
双歧杆菌益生菌对慢性牙周炎治疗的影响：一项随机临床试验。
影响因子：
6.7
作者：
Invernici MM;Salvador SL;Silva PHF;Soares MSM;Casarin R;Palioto DB;Souza SLS;Taba M Jr;Novaes AB Jr;Furlaneto FAC;Messora MR
通讯作者：
Messora MR
影响因子：
4.2
作者：
Engevik MA;Danhof HA;Hall A;Engevik KA;Horvath TD;Haidacher SJ;Hoch KM;Endres BT;Bajaj M;Garey KW;Britton RA;Spinler JK;Haag AM;Versalovic J
通讯作者：
Versalovic J