The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal.
The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal.
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DOI：
10.1186/s12866-021-02166-6
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发表时间：
2021-05-24
期刊：
影响因子：
4.2
通讯作者：
Versalovic J
中科院分区：
文献类型：
--
作者：
Engevik MA;Danhof HA;Hall A;Engevik KA;Horvath TD;Haidacher SJ;Hoch KM;Endres BT;Bajaj M;Garey KW;Britton RA;Spinler JK;Haag AM;Versalovic J
双歧杆菌是哺乳动物胃肠道的共生微生物。在这项研究中，我们的目的是确定齿状双歧杆菌的肠道定植机制和关键代谢途径。 B. 锷表现出耐酸性，在 4 至 7 的 pH 范围内具有高活力；与 B. dentium 基因组内 Na+/H+ 逆向转运蛋白的表达相关的发现。发现 B. dentium 粘附在人类 MUC2+ 粘液上并含有粘蛋白结合蛋白。使用微生物表型微阵列和完全限定的培养基，我们证明在没有葡萄糖的情况下，齿芽孢杆菌可以代谢多种营养源。其中许多营养来源都是植物性的，这表明齿芽孢杆菌可以消耗膳食物质。与其他双歧杆菌相比，齿双歧杆菌基本上无法在人类粘液中发现的化合物上生长。这一发现得到了其糖基水解酶 (GH) 谱的支持。通过蛋白质组学分析在齿芽孢杆菌中鉴定出的蛋白质中，大量蛋白质与不同的代谢途径相关，表明代谢可塑性支持动态胃肠道环境的定植。综上所述，我们得出结论，齿状芽孢杆菌非常适合在胃肠道中共生。在线版本包含可在 10.1186/s12866-021-02166-6 获取的补充材料。
Bifidobacteria are commensal microbes of the mammalian gastrointestinal tract. In this study, we aimed to identify the intestinal colonization mechanisms and key metabolic pathways implemented by Bifidobacterium dentium. B. dentium displayed acid resistance, with high viability over a pH range from 4 to 7; findings that correlated to the expression of Na+/H+ antiporters within the B. dentium genome. B. dentium was found to adhere to human MUC2+ mucus and harbor mucin-binding proteins. Using microbial phenotyping microarrays and fully-defined media, we demonstrated that in the absence of glucose, B. dentium could metabolize a variety of nutrient sources. Many of these nutrient sources were plant-based, suggesting that B. dentium can consume dietary substances. In contrast to other bifidobacteria, B. dentium was largely unable to grow on compounds found in human mucus; a finding that was supported by its glycosyl hydrolase (GH) profile. Of the proteins identified in B. dentium by proteomic analysis, a large cohort of proteins were associated with diverse metabolic pathways, indicating metabolic plasticity which supports colonization of the dynamic gastrointestinal environment. Taken together, we conclude that B. dentium is well adapted for commensalism in the gastrointestinal tract. The online version contains supplementary material available at 10.1186/s12866-021-02166-6.
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影响因子：
4.3
作者：
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通讯作者：
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影响因子：
2.5
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DOI：
10.1016/j.jcmgh.2020.08.002
发表时间：
2021
影响因子：
7.2
作者：
Engevik MA;Luck B;Visuthranukul C;Ihekweazu FD;Engevik AC;Shi Z;Danhof HA;Chang-Graham AL;Hall A;Endres BT;Haidacher SJ;Horvath TD;Haag AM;Devaraj S;Garey KW;Britton RA;Hyser JM;Shroyer NF;Versalovic J
通讯作者：
Versalovic J
影响因子：
3.6
作者：
Carvalho-Wells, Andrew L.;Helmolz, Kathrin;Tuohy, Kieran M.
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