Characterization and subcellular localization of histone deacetylases and their roles in response to abiotic stresses in soybean.
Characterization and subcellular localization of histone deacetylases and their roles in response to abiotic stresses in soybean.
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大豆组蛋白脱乙酰酶的表征和亚细胞定位及其在响应非生物胁迫中的作用
DOI：
10.1186/s12870-018-1454-7
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发表时间：
2018-10-11
影响因子：
5.3
通讯作者：
Luo M
中科院分区：
文献类型：
--
作者：
Yang C;Shen W;Chen H;Chu L;Xu Y;Zhou X;Liu C;Chen C;Zeng J;Liu J;Li Q;Gao C;Charron JB;Luo M
组蛋白去乙酰化酶（HDAC）是一种重要的表观遗传因子，在植物生长发育和植物对非生物或生物胁迫的反应中起着重要的调控作用。迄今为止，HDACs的分子功能在拟南芥中已得到了很好的描述，但在大豆（Glycine max）中还没有系统分析该基因家族的报道。系统发育分析表明，GmHDAC分为三个主要的组，以前命名为RPD 3/HDA 1，SIR 2和HD2。亚细胞定位分析表明，YFP标记的GmSRT 4、GmHDT 2和GmHDT 4主要定位于细胞核，而GmHDA 6、GmHDA 13、GmHDA 14和GmHDA 16则同时定位于细胞质和细胞核。实时荧光定量PCR结果表明，GmHDA 6、GmHDA 13、GmHDA 14、GmHDA 16和GmHDT 4在植物组织中广泛表达，而GmHDA 8、GmSRT 2、GmSRT 4和GmHDT 2在不同组织中表达差异。有趣的是，我们测量了不同的变化inGmHDAC转录积累响应几个非生物线索，表明这些表观遗传修饰可能是一个动态的转录响应大豆胁迫的一部分。最后，我们表明，以前报道的组蛋白标记与植物HDACs的水平调制由冷和热在此legume.ConclusionWe已确定和分类大豆HDACs基因28。我们的数据提供了深入了解的HDAC基因家族的进化，并进一步支持的假设，这些基因是重要的植物对环境胁迫的反应。
BackgroundHistone deacetylases (HDACs) function as key epigenetic factors in repressing the expression of genes in multiple aspects of plant growth, development and plant response to abiotic or biotic stresses. To date, the molecular function of HDACs is well described inArabidopsis thaliana, but no systematic analysis of this gene family in soybean (Glycine max) has been reported.ResultsIn this study, 28HDACgenes from soybean genome were identified, which were asymmetrically distributed on 12 chromosomes. Phylogenetic analysis demonstrated that GmHDACs fall into three major groups previously named RPD3/HDA1, SIR2, and HD2. Subcellular localization analysis revealed that YFP-tagged GmSRT4, GmHDT2 and GmHDT4 were predominantly localized in the nucleus, whereas GmHDA6, GmHDA13, GmHDA14 and GmHDA16 were found in both the cytoplasm and nucleus. Real-time quantitative PCR showed thatGmHDA6,GmHDA13,GmHDA14,GmHDA16andGmHDT4were broadly expressed across plant tissues, whileGmHDA8, GmSRT2,GmSRT4andGmHDT2showed differential expression across various tissues. Interestingly, we measured differential changes inGmHDACstranscripts accumulation in response to several abiotic cues, indicating that these epigenetic modifiers could potentially be part of a dynamic transcriptional response to stress in soybean. Finally, we show that the levels of histone marks previously reported to be associated with plant HDACs are modulated by cold and heat in this legume.ConclusionWe have identified and classified 28HDACgenes in soybean. Our data provides insights into the evolution of theHDACgene family and further support the hypothesis that these genes are important for the plant responses to environmental stress.
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类似稻草人的15与组蛋白脱乙酰基酶相互作用，对于抑制种子成熟程序至关重要。
影响因子：
16.6
作者：
Gao MJ;Li X;Huang J;Gropp GM;Gjetvaj B;Lindsay DL;Wei S;Coutu C;Chen Z;Wan XC;Hannoufa A;Lydiate DJ;Gruber MY;Chen ZJ;Hegedus DD
通讯作者：
Hegedus DD
影响因子：
7.4
作者：
Koenig, Ann-Christine;Hartl, Markus;Finkemeier, Iris
通讯作者：
Finkemeier, Iris
DOI：
10.1016/j.bbrc.2012.11.102
发表时间：
2013-03-08
影响因子：
3.1
作者：
Kim, Wanhui;Latrasse, David;Zhou, Dao-Xiu
通讯作者：
Zhou, Dao-Xiu
影响因子：
7.3
作者：
Buszewicz, Daniel;Archacki, Rafal;Koblowska, Marta Kamila
通讯作者：
Koblowska, Marta Kamila
番茄（Solanum lycopersicum）中九个组织特异性或应激反应性组蛋白脱乙酰酶基因的分子特征
影响因子：
4.8
作者：
Guo, Jun-E;Hu, Zongli;Chen, Guoping
通讯作者：
Chen, Guoping