Planarian fibroblast growth factor receptor homologs expressed in stem cells and cephalic ganglions
Planarian fibroblast growth factor receptor homologs expressed in stem cells and cephalic ganglions
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涡虫成纤维细胞生长因子受体同源物在干细胞和头神经节中表达
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
K. Agata
中科院分区：
文献类型：
--
作者：
K. Ogawa;C. Kobayashi;Tetsutaro Hayashi;H. Orii;Kenji Watanabe;K. Agata
涡虫强大的再生能力被认为存在于一种被称为“新胚细胞”的全能性体干细胞中。然而，调节干细胞分化/生长/迁移的信号系统仍不清楚。成纤维细胞生长因子（FGF）/FGF受体（FGFR）系统被认为在脊椎动物和无脊椎动物中都介导了各种发育过程。我们研究了DjFGFR1和DjFGFR2这两个与其他动物FGFR基因密切相关的涡虫基因的分子结构和表达情况。DjFGFR1和DjFGFR2蛋白在细胞外区域分别包含三个和两个免疫球蛋白样结构域，在细胞内区域包含一个分裂的酪氨酸激酶结构域。在完整的涡虫中，DjFGFR1和DjFGFR2在脑神经节和间充质空间中表达。在再生的涡虫中，在芽基以及再生咽或脑的片段中观察到表达DjFGFR1的细胞聚集。在失去再生能力的X射线照射的涡虫中，间充质空间中表达DjFGFR1的细胞数量显著减少。这些结果表明，DjFGFR1蛋白可能参与了控制涡虫再生的诸如干细胞分化/生长/迁移等方面的信号系统。
The strong regenerative capacity of planarians is considered to reside in the totipotent somatic stem cell called the ‘neoblast’. However, the signal systems regulating the differentiation/growth/migration of stem cells remain unclear. The fibroblast growth factor (FGF)/FGF receptor (FGFR) system is thought to mediate various developmental events in both vertebrates and invertebrates. We examined the molecular structures and expression of DjFGFR1 and DjFGFR2, two planarian genes closely related to other animal FGFR genes. DjFGFR1 and DjFGFR2 proteins contain three and two immunoglobulin‐like domains, respectively, in the extracellular region and a split tyrosine kinase domain in the intracellular region. Expression of DjFGFR1 and DjFGFR2 was observed in the cephalic ganglion and mesenchymal space in intact planarians. In regenerating planarians, accumulation of DjFGFR1‐expressing cells was observed in the blastema and in fragments regenerating either a pharynx or a brain. In X‐ray‐irradiated planarians, which had lost regenerative capacity, the number of DjFGFR1‐expressing cells in the mesenchymal space decreased markedly. These results suggest that the DjFGFR1 protein may be involved in the signal systems controlling such aspects of planarian regeneration as differentiation/growth/migration of stem cells.
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影响因子：
10.5
作者：
KLAMBT, C;GLAZER, L;SHILO, BZ
通讯作者：
SHILO, BZ
影响因子：
10.5
作者：
Sun, X;Meyers, EN;Martin, GR
通讯作者：
Martin, GR
DOI：
10.1073/pnas.87.11.4378
发表时间：
1990-06
影响因子：
11.1
作者：
A. Mansukhani;D. Moscatelli;D. Talarico;V. Levytska;C. Basilico
通讯作者：
A. Mansukhani;D. Moscatelli;D. Talarico;V. Levytska;C. Basilico
DOI：
10.1073/pnas.96.9.5049
发表时间：
1999-04-27
影响因子：
11.1
作者：
Alvarado, AS;Newmark, PA
通讯作者：
Newmark, PA
海胆 FGFR 肌肉特异性表达：胚胎和成体的转录后调控。
DOI：
10.1006/dbio.1998.8943
发表时间：
1998
期刊：
Developmental biology.
影响因子：
--
作者：
McCoon,PE;Blackstone,E;Angerer,RC;Angerer,LM
通讯作者：
Angerer,LM