Determination of optical markers of cyanobacterial physiology from fluorescence kinetics.
Determination of optical markers of cyanobacterial physiology from fluorescence kinetics.
复制标题
从荧光动力学中测定蓝细菌生理的光学标记。
DOI：
10.1093/plankt/fbac025
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发表时间：
2022-05
影响因子：
2.1
通讯作者：
中科院分区：
文献类型：
--
作者：
与其他监测和检测浮游植物中蓝藻的方法相比，荧光法提供了快速、可靠和可重复性的结果，并且可以在现场使用。能够提供生物量估计和生理信息的荧光计通常不是针对蓝藻进行优化的。这项研究详细概述了藻类和蓝藻培养物的荧光动力学，以确定针对所有浮游植物共同的或蓝藻诊断的荧光机制的最佳光学构型。我们证实，针对藻蓝蛋白和与光系统II相关的叶绿素a的荧光激发通道是诱导蓝藻荧光反应所必需的。此外，以660、685和730 nm为中心的发射通道允许更好地区分藻类和蓝藻培养物之间的荧光响应。蓝绿色光化光在蓝藻培养物中不产生强烈的荧光响应，应首选宽带光化光来评估环境光和光合作用之间的关系。蓝藻对光化光照射的强度和持续时间的荧光响应有显著的变异性，这在野外测量中需要考虑到。
Compared to other methods to monitor and detect cyanobacteria in phytoplankton populations, fluorometry gives rapid, robust and reproducible results and can be used in situ. Fluorometers capable of providing biomass estimates and physiological information are not commonly optimized to target cyanobacteria. This study provides a detailed overview of the fluorescence kinetics of algal and cyanobacterial cultures to determine optimal optical configurations to target fluorescence mechanisms that are either common to all phytoplankton or diagnostic to cyanobacteria. We confirm that fluorescence excitation channels targeting both phycocyanin and chlorophyll a associated to the Photosystem II are required to induce the fluorescence responses of cyanobacteria. In addition, emission channels centered at 660, 685 and 730 nm allow better differentiation of the fluorescence response between algal and cyanobacterial cultures. Blue-green actinic light does not yield a robust fluorescence response in the cyanobacterial cultures and broadband actinic light should be preferred to assess the relation between ambient light and photosynthesis. Significant variability was observed in the fluorescence response from cyanobacteria to the intensity and duration of actinic light exposure, which needs to be taken into consideration in field measurements.
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DOI：
10.1002/cyto.10104
发表时间：
2002-05-01
期刊：
CYTOMETRY
影响因子：
--
作者：
Becker, A;Meister, A;Wilhelm, C
通讯作者：
Wilhelm, C
影响因子：
3.7
作者：
Beutler, M;Wiltshire, KH;Dau, H
通讯作者：
Dau, H
影响因子：
4.4
作者：
Baker, JA;Entsch, B;McKay, DB
通讯作者：
McKay, DB
影响因子：
12.8
作者：
Gregor, Jakub;Marsalek, Blahoslav;Sipkova, Helena
通讯作者：
Sipkova, Helena
影响因子：
7.4
作者：
KOPF, U;HEINZE, J
通讯作者：
HEINZE, J