Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani.
Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani.
复制标题
黄粉虫蛹对瓜尼硬皮虫寄生的转录组免疫反应。
DOI：
10.1371/journal.pone.0054411
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yang B
中科院分区：
文献类型：
--
作者：
Zhu JY;Yang P;Zhang Z;Wu GX;Yang B
寄主与拟寄生蜂的相互作用是昆虫最有趣的关系之一，目前正受到越来越多的关注。了解寄生蜂进化的机制，以逃避或抑制宿主免疫系统是重要的解剖这种相互作用，而它仍然知之甚少。为了了解黄粉虫对管氏肿腿蜂寄生的免疫反应，对管氏肿腿蜂T。对黄粉虫免疫相关基因进行了测序，并对未寄生和寄生的黄粉虫进行了比较。通过数字基因表达（DGE）分析，特别强调使用Illumina测序的寄生虫诱导的免疫相关基因。在单次运行中，获得了264，698个原始读数。从头组装产生71，514个单基因，平均长度为424 bp。在这些单基因中，37，373个（52.26%）与NCBI nr数据库中的已知蛋白质相似。通过对转录组数据的深入分析，确定了430个与免疫相关的单基因。DGE分析表明，S. guani对T.黄粉虫，如3，431寄生反应转录物的显著上调或下调所示。共检测到74个与T.黄粉虫在寄生后发生了显著变化。获得了T.黄粉虫转录组除了为进一步研究功能基因组学建立了基础资源外，还发现了大量免疫基因，这些基因可能为更好地了解该物种和其他甲虫的免疫反应提供了有意义的框架。DGE分析数据提供了全面的T。在转录水平上的免疫基因的表达信息，寄生后，并提供了有价值的光的分子理解的主机-寄生虫的相互作用。
Host and parasitoid interaction is one of the most fascinating relationships of insects, which is currently receiving an increasing interest. Understanding the mechanisms evolved by the parasitoids to evade or suppress the host immune system is important for dissecting this interaction, while it was still poorly known. In order to gain insight into the immune response of Tenebrio molitor to parasitization by Scleroderma guani, the transcriptome of T. molitor pupae was sequenced with focus on immune-related gene, and the non-parasitized and parasitized T. molitor pupae were analyzed by digital gene expression (DGE) analysis with special emphasis on parasitoid-induced immune-related genes using Illumina sequencing. In a single run, 264,698 raw reads were obtained. De novo assembly generated 71,514 unigenes with mean length of 424 bp. Of those unigenes, 37,373 (52.26%) showed similarity to the known proteins in the NCBI nr database. Via analysis of the transcriptome data in depth, 430 unigenes related to immunity were identified. DGE analysis revealed that parasitization by S. guani had considerable impacts on the transcriptome profile of T. molitor pupae, as indicated by the significant up- or down-regulation of 3,431 parasitism-responsive transcripts. The expression of a total of 74 unigenes involved in immune response of T. molitor was significantly altered after parasitization. obtained T. molitor transcriptome, in addition to establishing a fundamental resource for further research on functional genomics, has allowed the discovery of a large group of immune genes that might provide a meaningful framework to better understand the immune response in this species and other beetles. The DGE profiling data provides comprehensive T. molitor immune gene expression information at the transcriptional level following parasitization, and sheds valuable light on the molecular understanding of the host-parasitoid interaction.
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影响因子：
3.7
作者：
Bai X;Mamidala P;Rajarapu SP;Jones SC;Mittapalli O
通讯作者：
Mittapalli O
影响因子：
3.7
作者：
Fang Q;Wang F;Gatehouse JA;Gatehouse AM;Chen XX;Hu C;Ye GY
通讯作者：
Ye GY
影响因子：
4.4
作者：
Barat-Houari M;Hilliou F;Jousset FX;Sofer L;Deleury E;Rocher J;Ravallec M;Galibert L;Delobel P;Feyereisen R;Fournier P;Volkoff AN
通讯作者：
Volkoff AN
影响因子：
3.6
作者：
Dziarski, R
通讯作者：
Dziarski, R
DOI：
10.1111/j.1432-1033.1997.00614.x
发表时间：
1997-07-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Axen, A;Carlsson, A;Bennich, H
通讯作者：
Bennich, H