Broad Cross-Reactive IgA and IgG against Human Coronaviruses in Milk Induced by COVID-19 Vaccination and Infection.
Broad Cross-Reactive IgA and IgG against Human Coronaviruses in Milk Induced by COVID-19 Vaccination and Infection.
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DOI：
10.3390/vaccines10060980
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发表时间：
2022-06-20
期刊：
影响因子：
7.8
通讯作者：
Zand, Martin S.
中科院分区：
文献类型：
--
作者：
Wang, Jiong;Young, Bridget E.;Li, Dongmei;Seppo, Antti;Zhou, Qian;Wiltse, Alexander;Nowak-Wegrzyn, Anna;Murphy, Katherine;Widrick, Kaili;Diaz, Nicole;Cruz-Vasquez, Joseline;M. Jarvinen, Kirsi;Zand, Martin S.
关键词：
目前尚不清楚SARS-CoV-2感染或mRNA疫苗接种是否也能在哺乳期父母体内诱导抗常见人类冠状病毒(HCoV)的Ig G和Ig A。在这里，我们前瞻性地分析了母乳(HM)和哺乳期父母的血液样本，以测量抗SARS-CoV-2特异性和抗HCoV交叉反应的IgA和Ig G反应的时间模式。分析了两个队列：接受新冠肺炎(MRNA-1273或BNT162b2)基因疫苗的接种队列(n=30)和感染新冠肺炎的队列(n=45)。在疫苗接种前和接种后，或对于感染者，在确诊诊断后14-28天的5个时间点采集纵向HM和指棒血样。用多重免疫分析法(mPlex-CoV)检测血清中抗SARS-CoV-2和抗-HCoV的抗体水平(S)和抗核衣壳抗体(N)。结果发现，接种疫苗后HM中抗S免疫球蛋白A和Ig G水平显著升高。相比之下，在第二次接种疫苗后，虽然免疫球蛋白水平上升，但血液和HM IgA开始下降。此外，HM与血中抗S抗体Ig G水平显著相关，而抗S Ig A水平无显著相关性。SARS-2急性感染可产生抗S抗体，与疫苗接种相比，HM与血液的相关性更高。接种疫苗和感染能够显著增加HM和血液中识别HCoV的广谱交叉反应免疫球蛋白G，而不是HM和血液中的IgA抗体。此外，新冠肺炎免疫球蛋白与血液中的免疫球蛋白具有较广泛的交叉反应性，提示接种HM疫苗和感染可能通过HM为母乳喂养的婴儿提供被动免疫，不仅对SARS-CoV-2，而且对普通感冒冠状病毒也是如此。
It is currently unclear if SARS-CoV-2 infection or mRNA vaccination can also induce IgG and IgA against common human coronaviruses (HCoVs) in lactating parents. Here we prospectively analyzed human milk (HM) and blood samples from lactating parents to measure the temporal patterns of anti-SARS-CoV-2 specific and anti-HCoV cross-reactive IgA and IgG responses. Two cohorts were analyzed: a vaccination cohort (n = 30) who received mRNA-based vaccines for COVID-19 (mRNA-1273 or BNT162b2), and an infection cohort (n = 45) with COVID-19 disease. Longitudinal HM and fingerstick blood samples were collected pre- and post-vaccination or, for infected subjects, at 5 time-points 14–28 days after confirmed diagnosis. The anti-spike(S) and anti-nucleocapsid(N) IgA and IgG antibody levels against SARS-CoV-2 and HCoVs were measured by multiplex immunoassay (mPlex-CoV). We found that vaccination significantly increased the anti-S IgA and IgG levels in HM. In contrast, while IgG levels increased after a second vaccine dose, blood and HM IgA started to decrease. Moreover, HM and blood anti-S IgG levels were significantly correlated, but anti-S IgA levels were not. SARS2 acute infection elicited anti-S IgG and IgA that showed much higher correlations between HM and blood compared to vaccination. Vaccination and infection were able to significantly increase the broadly cross-reactive IgG recognizing HCoVs in HM and blood than the IgA antibodies in HM and blood. In addition, the broader cross-reactivity of IgG in HM versus blood indicates that COVID-19 vaccination and infection might provide passive immunity through HM for the breastfed infants not only against SARS-CoV-2 but also against common cold coronaviruses.
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影响因子：
5.8
作者：
Fox A;Marino J;Amanat F;Krammer F;Hahn-Holbrook J;Zolla-Pazner S;Powell RL
通讯作者：
Powell RL
DOI：
10.1053/siny.2002.0124
发表时间：
2002-08-01
期刊：
Seminars in neonatology : SN
影响因子：
--
作者：
Hanson, Lars A;Korotkova, Marina
通讯作者：
Korotkova, Marina
影响因子：
9.1
作者：
Hicks J;Klumpp-Thomas C;Kalish H;Shunmugavel A;Mehalko J;Denson JP;Snead KR;Drew M;Corbett KS;Graham BS;Hall MD;Memoli MJ;Esposito D;Sadtler K
通讯作者：
Sadtler K
影响因子：
13.2
作者：
Guo L;Wang Y;Kang L;Hu Y;Wang L;Zhong J;Chen H;Ren L;Gu X;Wang G;Wang C;Dong X;Wu C;Han L;Wang Y;Fan G;Zou X;Li H;Xu J;Jin Q;Cao B;Wang J
通讯作者：
Wang J
影响因子：
2.6
作者：
Baird, Jill K.;Jensen, Shawn M.;Baird, Jason R.
通讯作者：
Baird, Jason R.