H3N2 canine influenza virus and Enterococcus faecalis coinfection in dogs in China
H3N2 canine influenza virus and Enterococcus faecalis coinfection in dogs in China
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中国犬 H3N2 犬流感病毒和粪肠球菌双重感染
DOI：
10.1186/s12917-019-1832-x
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发表时间：
2019-04
影响因子：
2.6
通讯作者：
Yanli Lyu
中科院分区：
文献类型：
--
作者：
Liwei Zhou;Haoran Sun;Shikai Song;Jinhua Liu;Zhaofei Xia;Yipeng Sun;Yanli Lyu
背景：2017年5月，中国北方某德国牧羊犬饲养场内的17只犬出现呼吸道临床症状。主人给狗狗静脉注射双黄连中药和阿奇霉素。然而，治疗后3天，呼吸道症状有所改善。8只狗的颈部出现囊肿，其中3只在随后的2天内死亡。病例介绍：采用实时定量PCR检测犬流感病毒（CIV）。该犬舍中的所有狗均呈阳性，其余 14 只狗已发生血清转化。其中两只狗被送往中国农业大学兽医教学医院接受进一步检查。从这些狗的鼻拭子中分离出两种流感病毒株（A/canine/.Beijing/0512–133/2017 和 A/canine/Beijing/0512–137/2017），经测序并鉴定为禽源 H3N2 CIV。入院的两只狗的血液学检查显示有轻度炎症，X光检查结果显示肺炎。将包囊液铺板用于细菌培养，并进行细菌16s rRNA基因PCR，随后进行Sanger测序。结果表明粪肠球菌感染。进行了抗菌药物敏感性测试，并用恩诺沙星治疗狗。其余14只狗均在16天内康复。结论：狗体内检测到H3N2 CIV和粪肠球菌双重感染，此前未有报道。我们的研究结果强调，CIV 感染可能会促进机会性细菌的继发感染，并导致更严重和复杂的临床结果。
Background: In May 2017, 17 dogs in a German Shepherd breeding kennel in northern China developed.respiratory clinical signs. The owner treated the dogs with an intravenous injection of Shuang-Huang-lian, a.traditional Chinese medicine, and azithromycin. The respiratory signs improved 3 days post-treatment, however,.cysts were observed in the necks of eight dogs, and three of them died in the following 2 days..Case presentation: Quantitative real-time PCR was used to detect canine influenza virus (CIV). All of the dogs in.this kennel were positive and the remaining 14 dogs had seroconverted. Two of the dogs were taken to the China.Agricultural University Veterinary Teaching Hospital for further examination. Two strains of influenza virus (A/canine/.Beijing/0512–133/2017 and A/canine/Beijing/0512–137/2017) isolated from the nasal swabs of these dogs were.sequenced and identified as avian-origin H3N2 CIV. For the two dogs admitted to the hospital, hematology showed.mild inflammation and radiograph results indicated pneumonia. Cyst fluid was plated for bacterial culture and.bacterial 16 s rRNA gene PCR was performed, followed by Sanger sequencing. The results indicated an Enterococcus.faecalis infection. Antimicrobial susceptibility tests were performed and dogs were treated with enrofloxacin. All 14.remaining dogs recovered within 16 days..Conclusions: Coinfection of H3N2 CIV and Enterococcus faecalis was detected in dogs, which has not been.reported previously. Our results highlight that CIV infection might promote the secondary infection of opportunistic.bacteria and cause more severe and complicated clinical outcomes..
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影响因子：
1.4
作者：
Sid, Hicham;Benachour, Karine;Rautenschlein, Silke
通讯作者：
Rautenschlein, Silke
DOI：
10.1016/j.meegid.2010.08.010
发表时间：
2010-12
期刊：
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
影响因子：
--
作者：
Li S;Shi Z;Jiao P;Zhang G;Zhong Z;Tian W;Long LP;Cai Z;Zhu X;Liao M;Wan XF
通讯作者：
Wan XF
影响因子：
2.6
作者：
Pietschmann J;Mur L;Blome S;Beer M;Pérez-Sánchez R;Oleaga A;Sánchez-Vizcaíno JM
通讯作者：
Sánchez-Vizcaíno JM
影响因子：
3.3
作者：
Teng, Qiaoyang;Zhang, Xu;Li, Zejun
通讯作者：
Li, Zejun
影响因子：
2.4
作者：
Watson, C. E.;Bell, C.;Toohey-Kurth, K.
通讯作者：
Toohey-Kurth, K.