Ultra-Sensitive and Semi-Quantitative Vertical Flow Assay for the Rapid Detection of Interleukin-6 in Inflammatory Diseases.
Ultra-Sensitive and Semi-Quantitative Vertical Flow Assay for the Rapid Detection of Interleukin-6 in Inflammatory Diseases.
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DOI：
10.3390/bios12090756
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发表时间：
2022-09-14
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炎症生物标志物白介素6(IL-6)在健康血清中的浓度低于7pg/mL，但在发生炎症时浓度会增加10-100倍。据报道，在类风湿性关节炎(RA)等慢性疾病以及脓毒症和细胞因子释放综合征(CRS)等危及生命的急性疾病中，血清IL-6水平升高。这项工作旨在满足血清IL-6快速检测的需求，既用于慢性病的快速监测，也用于急性病患者的分诊。在对几种类型的金纳米粒子、膜孔径和缓冲体系进行优化后，设计了一种超灵敏的垂直流动分析(VFA)，可以检测添加到缓冲液中的重组IL-6，检测限(LOD)为10 pg/mL，报告范围为10-10,000 pg/mL，检测时间为15分钟。检测健康血清中IL-6的最低检测限为3.2pg/mL，报告范围为10-10000 pg/mL。在室温下，VFA在1天、2周、4周和6周的保存期内表现出了稳定性。术中、术中变异系数分别为14.3%和15.2%。在试剂盒中引入了三个参照区，高、低和空白，以便于在6点半定量范围内进行现场半定量测量。最后，以EL ISA为金标准平台，用20例RA和20例健康对照(HC)临床血清标本对IL-6 VFA的性能进行了验证。超灵敏、快速的IL-6 VFA可能被用于对患者进行分诊，进行重症监护、治疗调整，或用于监测炎症条件下的疾病活动。
The inflammation biomarker Interleukin 6 (IL-6) exhibits a concentration of less than 7 pg/mL in healthy serum but increases 10–100-fold when inflammation occurs. Increased serum IL-6 has been reported in chronic diseases such as rheumatoid arthritis (RA), as well as in life-threatening acute illnesses such as sepsis and cytokine release syndrome (CRS). This work seeks to meet the demand for rapid detection of serum IL-6 both for rapid monitoring of chronic diseases and for triaging patients with acute illnesses. Following the optimization of several types of gold nanoparticles, membrane pore sizes, and buffer systems, an ultra-sensitive vertical flow assay (VFA) was engineered, allowing the detection of recombinant IL-6 in spiked buffer with a limit of detection (LoD) of 10 pg/mL and a reportable range of 10–10,000 pg/mL with a 15-min assay time. The detection of IL-6 in spiked pooled healthy serum exhibited an LoD of 3.2 pg/mL and a reportable range of 10–10,000 pg/mL. The VFA’s stability was demonstrated over 1-day, two-week, four-week, and six-week storage durations at room temperature. The inter-operator CV and intra-operator CV were determined to be 14.3% and 15.2%, respectively. Three reference zones, high, low, and blank, were introduced into the cartridge to facilitate on-site semi-quantitative measurements across a 6-point semi-quantitative range. Finally, the performance of the IL-6 VFA was validated using 20 RA and 20 healthy control (HC) clinical serum samples, using ELISA as the gold standard platform. The ultra-sensitive, rapid IL-6 VFA could potentially be used to triage patients for intensive care, treatment adjustments, or for monitoring disease activity in inflammatory conditions.
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影响因子：
0.9
作者：
Helal, Abdel Moneim H.;Shahine, Enas M.;Moneim, Riham Abdel
通讯作者：
Moneim, Riham Abdel
DOI：
10.3390/diagnostics12051107
发表时间：
2022-04-28
期刊：
Diagnostics (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
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影响因子：
4.6
作者：
Park YJ;Yoo SA;Kim GR;Cho CS;Kim WU
通讯作者：
Kim WU
影响因子：
3.8
作者：
Mirza, S.;Hossain, Monir;Mathews, Christine;Martinez, Perla;Pino, Paula;Gay, Jennifer L.;Rentfro, Anne;McCormick, Joseph B.;Fisher-Hoch, Susan P.
通讯作者：
Fisher-Hoch, Susan P.
影响因子：
33.7
作者：
Choy, Ernest H.;De Benedetti, Fabrizio;Kishimoto, Tadamitsu
通讯作者：
Kishimoto, Tadamitsu