In vivo skin penetration of quantum dot nanoparticles in the murine model: the effect of UVR.
In vivo skin penetration of quantum dot nanoparticles in the murine model: the effect of UVR.
复制标题
DOI：
10.1021/nl801323y
复制
发表时间：
2008-09
期刊：
影响因子：
10.8
通讯作者：
Delouise LA
中科院分区：
文献类型：
--
作者：
Mortensen LJ;Oberdörster G;Pentland AP;Delouise LA
紫外线辐射（UVR）对皮肤屏障的生物学和完整性具有广泛的影响。自20世纪80年代以来，一直在研究驱动这些变化的机制及其对皮肤屏障功能的影响。然而，没有研究检查紫外线对纳米粒子皮肤渗透的影响。纳米颗粒（NP）通常用于防晒霜和其他化妆品中，由于消费者使用的防晒霜通常应用于阳光受损的皮肤，因此由于潜在的毒性，UVR对NP皮肤渗透的影响是一个问题。在这项研究中，我们调查纳米粒子皮肤渗透采用在体内半导体量子点纳米粒子（QD）模型系统。该模型系统提高了NP成像能力，并提供了额外的主要利益，由于广泛和扩大使用的量子点在研究应用和制造。在我们的实验中，在有和没有UVR暴露的情况下，在甘油载体中将羧化QD应用于SKH-1小鼠的皮肤。QD给药后8小时和24小时，使用组织组织学、共聚焦显微镜、透射电子显微镜（TEM）和EDAX分析评价皮肤收集和渗透模式。在未暴露于UVR的小鼠和暴露于UVR的小鼠中均观察到低水平的渗透。在暴露于UVR的小鼠中可观察到更高水平的穿透。这些结果是第一次在体内QD皮肤渗透，并提供了重要的洞察QD穿透完整的和UVR受损的皮肤屏障的能力。我们的研究结果引起了人们的关注，即类似大小和表面化学性质的NP，如防晒霜中发现的金属氧化物NP，也可能渗透紫外线损伤的皮肤。
Ultraviolet radiation (UVR) has widespread effects on the biology and integrity of the skin barrier. Research on the mechanisms that drive these changes, as well as their effect on skin barrier function has been ongoing since the 1980s. However, no studies have examined the impact of UVR on nanoparticle skin penetration. Nanoparticles (NP) are commonly used in sunscreens and other cosmetics, and since consumer use of sunscreen is often applied to sun damaged skin, the effect of UVR on NP skin penetration is a concern due to potential toxicity. In this study we investigate nanoparticle skin penetration by employing an in vivo semiconductor quantum dot nanoparticle (QD) model system. This model system improves NP imaging capabilities and provides additional primary interest due to widespread and expanding use of QD in research applications and manufacturing. In our experiments, carboxylated QD were applied to the skin of SKH-1 mice in a glycerol vehicle with and without UVR exposure. The skin collection and penetration patterns were evaluated 8 and 24 hours after QD application using tissue histology, confocal microscopy, and transmission electron microscopy (TEM) and EDAX analysis. Low levels of penetration were seen in both the non-UVR exposed mice and the UVR exposed mice. Qualitatively higher levels of penetration were observable in the UVR exposed mice. These results are the first for in vivo QD skin penetration, and provide important insight into the ability of QD to penetrate intact and UVR compromised skin barrier. Our findings raise concern that NP of similar size and surface chemistry, such as metal oxide NP found in sunscreens, may also penetrate UV damaged skin.
登录
查看更多内容
影响因子：
4.6
作者：
Jiang, Shao Jun;Chen, Jie Yu;Zhou, Xiao Jun
通讯作者：
Zhou, Xiao Jun
影响因子：
10.4
作者：
Hardman R
通讯作者：
Hardman R
DOI：
10.1073/pnas.152463399
发表时间：
2002-10-01
影响因子：
11.1
作者：
Åkerman, ME;Chan, WCW;Ruoslahti, E
通讯作者：
Ruoslahti, E
影响因子：
4.5
作者：
Gurr, JR;Wang, ASS;Jan, KY
通讯作者：
Jan, KY
影响因子：
13.3
作者：
Chang, Emmanuel;Thekkek, Nadhi;Drezek, Rebekah
通讯作者：
Drezek, Rebekah