Label-free detection of cardiac troponin I with a photonic crystal biosensor.
Label-free detection of cardiac troponin I with a photonic crystal biosensor.
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DOI：
10.1016/j.bios.2014.02.057
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发表时间：
2014-08-15
影响因子：
12.6
通讯作者：
Ye, Jing Yong
中科院分区：
文献类型：
--
作者：
Zhang, Bailin;Morales, Andres W.;Peterson, Ralph;Tang, Liang;Ye, Jing Yong
开发了一种用于全内反射(PC-TIR)配置的具有光子晶体结构的生物传感器，用于心脏生物标记物肌钙蛋白I(CTnI)的无标记检测。与传统的光学微腔不同，传统的光学微腔具有封闭结构，其腔层夹在两个高反射表面之间，PC-TIR配置创建了独特的开放式微腔，允许其腔层(感测层)易于功能化并直接暴露于生物分析的分析物分子。在这项研究中，PC-TIR传感器已经被用于通过监测由于生物分子结合过程而引起的腔的共振条件的变化来进行心脏生物标志物的无标记测量。针对cTnI的抗体被固定在传感器表面，用于在广泛的浓度范围内特异性地检测cTnI。CTnI的检出限可达0.1ngmL−-1。
A biosensor has been developed with a photonic crystal structure used in a total-internal-reflection (PC-TIR) configuration for label-free detection of a cardiac biomarker: Troponin I (cTnI). In contrast to a conventional optical microcavity that has a closed structure with its cavity layer sandwiched between two high-reflection surfaces, the PC-TIR configuration creates a unique open microcavity, which allows its cavity layer (sensing layer) to be easily functionalized and directly exposed to analyte molecules for bioassays. In this study, a PC-TIR sensor has been used for the label-free measurements of cardiac biomarkers by monitoring the changes in the resonant condition of the cavity due to biomolecular binding processes. Antibodies against cTnI are immobilized on the sensor surface for specific detection of cTnI with a wide range of concentrations. Detection limit of cTnI with a concentration as low as 0.1 ng mL−1 has been achieved.
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DOI：
10.1109/jstqe.2005.862945
发表时间：
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影响因子：
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