Effects of Aging on Human Meibum.
Effects of Aging on Human Meibum.
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DOI：
10.1167/iovs.62.12.23
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发表时间：
2021-09-02
影响因子：
4.4
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Butovich IA;Suzuki T
本研究的目的是确定年龄是否会影响非睑板腺功能障碍(MGD)/非干眼症（DE）人群的脂质组成。在许多临床和人群调查中，衰老一再与各种组织和器官的病理变化有关，包括MGD和DE的发病。除其他因素外，这两种情况都与代谢异常分泌和组成有关。然而，这种联系的化学基础尚未建立。为了确定和表征随着年龄增长而可能发生的减数代谢和减数发生的变化，我们对两个年龄组——年轻人（29±5岁，n = 21）和老年人（68±7岁，n = 29）收集的减数代谢样本进行了脂质组学分析，每组的男女比例相似。采用脂质体范围内的非靶向（如主成分分析）和靶向（如潜在结构的正交投影判别分析）方法，以及液相色谱质谱（LC-MS）和串联质谱（MS-MS）实验对特定脂质进行重点分析，比较了脂肪主要脂类（如蜡酯、胆固醇酯、游离胆固醇、三酰基甘油等）的完整脂质种类。在两个年龄组中观察到非常高的脂质相似性，在单个脂质种类中只有微小的变化。所测试的脂质种类的组间变异性的大小与相同代谢成分的组内变异性相当。在脂质酯化、延伸和不饱和模式上没有统计学上的显著差异。在健康、非mgd /非de受试者中，时间顺序衰老本身似乎对meibogenesis只有很小的影响。
The purpose of this study was to determine if aging affects meibum lipid composition in non-meibomian gland dysfunction (MGD)/non-dry eye (DE) population. Aging has been repeatedly linked to pathological changes in various tissues and organs, including the onset of MGD and DE, in a number of clinical and population-wide surveys. Both conditions have been associated with abnormal meibum secretion and composition, among other factors. However, the chemical basis for such a connection has not been established yet. To identify and characterize possible changes in the meibum and meibogenesis with aging, lipidomic analyses of meibum samples collected from human subjects of two age groups – young (29 ± 5 years, n = 21) and elderly (68 ± 7 years, n = 29) – with similar male to female ratios in each group were conducted. Intact lipid species from major lipid groups of meibum (such as wax esters, cholesteryl esters, free cholesterol, triacylglycerols, etc.) were compared using lipidome-wide untargeted (such as Principal Component Analysis) and targeted (such as Orthogonal Projections to Latent Structures Discriminant Analysis) approaches, along with focused analyses of specific lipid species in liquid-chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS-MS) experiments. Extremely high similarities of meibum lipids in the two age groups were observed, with only minor changes in the individual lipid species. The magnitude of the intergroup variability for tested lipid species was comparable to the intragroup variability for the same meibum components. No statistically significant differences in the lipid esterification, elongation, and unsaturation patterns were observed. Chronological aging itself seems to have only minor effect on meibogenesis in healthy, non-MGD/non-DE subjects.
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影响因子：
4.4
作者：
Amano, Shiro
通讯作者：
Amano, Shiro
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通讯作者：
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作者：
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通讯作者：
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作者：
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通讯作者：
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作者：
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通讯作者：
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