Mis-spliced transcripts of nicotinic acetylcholine receptor alpha6 are associated with field evolved spinosad resistance in Plutella xylostella (L.).
Mis-spliced transcripts of nicotinic acetylcholine receptor alpha6 are associated with field evolved spinosad resistance in Plutella xylostella (L.).
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DOI：
10.1371/journal.pgen.1000802
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发表时间：
2010-01
期刊：
影响因子：
4.5
通讯作者：
Jiggins CD
中科院分区：
文献类型：
--
作者：
Baxter SW;Chen M;Dawson A;Zhao JZ;Vogel H;Shelton AM;Heckel DG;Jiggins CD
杀虫剂抗药性的演变是全球农业生产的制约因素。多杀菌素是一种新的、低环境风险的杀虫剂，主要针对烟碱乙酰胆碱受体（nAChR），对多种害虫有效。然而，在仅仅几年的应用之后，在世界各地的芸苔作物的重要害虫小菜蛾（Plutella xylostella）中出现了田间进化抗性。多杀菌素抗性在夏威夷人口的结果从一个单一的不完全隐性和常染色体基因，在这里，我们使用AFLP连锁图谱，以确定染色体控制抗性回交家庭。对700多个回交后代进行的连锁作图定位了一个假定的多杀菌素靶标nAChR α 6（Pxα6），位于抗性基因座PxSpinR。Pxα6的第九内含子剪接点内的突变导致转录本的错误剪接，从而产生在第三和第四跨膜结构域之间截短的预测蛋白。检测到排除含突变外显子的其他耐药相关Pxα6转录物，预测这些转录物也会产生截短蛋白。鉴定这一重要作物害虫的抗性位点将有助于田间监测抗性的传播，并提供对其他物种多杀菌素抗性遗传基础的深入了解。对抗生素或杀虫剂等控制剂的抗药性的发展可能对人类健康或农业食品生产造成重大损失。一旦确定了对特定化合物的抗性的遗传机制，就可以迅速评估其他抗性物种，以寻找类似的机制。杀虫剂通常以昆虫的神经系统为目标，因为它们在低浓度下可能是有毒的，并且作用迅速。在这里，我们报告了一种全球性农业害虫小菜蛾的基因突变，这种突变与对生物杀虫剂多杀菌素的抗性有关。烟碱乙酰胆碱受体（nAChR）α 6内含子剪接点的突变导致错误剪接的mRNA转录本，预计会产生缺乏重要功能结构域的截短蛋白。nAChR需要5个亚基才能发挥功能，昆虫通常编码10-12个亚基基因。因此，多杀菌素可能是针对一个多余的乙酰胆碱受体亚基不必要的生存小菜蛾。现在可以测试其他对多杀菌素产生田间抗性的昆虫，以确定是否涉及相同的抗性机制。
The evolution of insecticide resistance is a global constraint to agricultural production. Spinosad is a new, low-environmental-risk insecticide that primarily targets nicotinic acetylcholine receptors (nAChR) and is effective against a wide range of pest species. However, after only a few years of application, field evolved resistance emerged in the diamondback moth, Plutella xylostella, an important pest of brassica crops worldwide. Spinosad resistance in a Hawaiian population results from a single incompletely recessive and autosomal gene, and here we use AFLP linkage mapping to identify the chromosome controlling resistance in a backcross family. Recombinational mapping with more than 700 backcross progeny positioned a putative spinosad target, nAChR alpha 6 (Pxα6), at the resistance locus, PxSpinR. A mutation within the ninth intron splice junction of Pxα6 results in mis-splicing of transcripts, which produce a predicted protein truncated between the third and fourth transmembrane domains. Additional resistance-associated Pxα6 transcripts that excluded the mutation containing exon were detected, and these were also predicted to produce truncated proteins. Identification of the locus of resistance in this important crop pest will facilitate field monitoring of the spread of resistance and offer insights into the genetic basis of spinosad resistance in other species. Evolving resistance to control agents, such as antibiotics or insecticides, can have major costs to human health or agricultural food production. Once a genetic mechanism for resistance to a particular compound has been identified, other resistant species can be rapidly assessed to search for a parallel mechanism. Insecticides often target the insect nervous system as they can be toxic at low concentration and act rapidly. Here we report a genetic mutation in a global agricultural pest, diamondback moth, that is associated with resistance to the bioinsecticide spinosad. A mutation in an intron splice junction of nicotinic acetylcholine receptor (nAChR) alpha 6 causes mis-spliced mRNA transcripts that are predicted to produce truncated proteins lacking important functional domains. nAChRs require 5 subunits to function, and insects generally encode 10–12 subunit genes. Spinosad may therefore be targeting a redundant nAChR subunit not essential for survival in diamondback moth. Other insects that evolve field resistance to spinosad can now be tested to determine whether the same resistance mechanism is involved.
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红粉甲虫的cys-loop配体门控离子通道基因tribolium castaneum。
影响因子：
4.4
作者：
Jones, Andrew K;Sattelle, David B
通讯作者：
Sattelle, David B
DOI：
10.1073/pnas.0831036100
发表时间：
2003-04-29
影响因子：
11.1
作者：
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通讯作者：
Tabashnik, BE
影响因子：
3.4
作者：
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通讯作者：
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影响因子：
1.9
作者：
Bond, JG;Marina, CF;Williams, T
通讯作者：
Williams, T
影响因子：
4.4
作者：
Jones, AK;Grauso, M;Sattelle, DB
通讯作者：
Sattelle, DB