Cell-cycle regulated transcription associates with DNA replication timing in yeast and human.
Cell-cycle regulated transcription associates with DNA replication timing in yeast and human.
复制标题
DOI：
10.1186/gb-2013-14-10-r111
复制
发表时间：
2013
期刊：
影响因子：
12.3
通讯作者：
Fraser HB
中科院分区：
文献类型：
--
作者：
Fraser HB
真核 DNA 复制遵循特定的时间程序，某些基因组区域始终比其他区域更早复制，但决定该程序的因素很大程度上未知。迄今为止，在所有研究的植物和动物物种中，已观察到高度转录的区域在早期 S 期复制，但这种关系被认为在芽殖酵母和裂殖酵母中都不存在。对于任何物种，细胞周期调节的转录和复制时间之间没有关联的报道。在这里，我展示了在出芽酵母、裂殖酵母和人类中，S 期转录程度最高的基因复制较早，而那些在 S 期抑制的基因复制较晚。其他细胞周期阶段的转录要么与复制时间呈现相反的相关性，要么没有关系。这种关系在复制的晚激发起点附近最强，这与之前提出的模型（复制时间可能影响转录）不一致，而是表明了一种潜在机制，涉及在 S 期招募限制性复制起始因子。这些结果表明，S 期转录可能是真核生物 DNA 复制时间的重要决定因素，这可以解释转录和复制时间之间既定的关联。
Eukaryotic DNA replication follows a specific temporal program, with some genomic regions consistently replicating earlier than others, yet what determines this program is largely unknown. Highly transcribed regions have been observed to replicate in early S-phase in all plant and animal species studied to date, but this relationship is thought to be absent from both budding yeast and fission yeast. No association between cell-cycle regulated transcription and replication timing has been reported for any species. Here I show that in budding yeast, fission yeast, and human, the genes most highly transcribed during S-phase replicate early, whereas those repressed in S-phase replicate late. Transcription during other cell-cycle phases shows either the opposite correlation with replication timing, or no relation. The relationship is strongest near late-firing origins of replication, which is not consistent with a previously proposed model—that replication timing may affect transcription—and instead suggests a potential mechanism involving the recruitment of limiting replication initiation factors during S-phase. These results suggest that S-phase transcription may be an important determinant of DNA replication timing across eukaryotes, which may explain the well-established association between transcription and replication timing.
登录
查看更多内容
影响因子：
56.9
作者：
Raghuraman, MK;Winzeler, EA;Fangman, WL
通讯作者：
Fangman, WL
影响因子：
14.9
作者：
Gauthier NP;Jensen LJ;Wernersson R;Brunak S;Jensen TS
通讯作者：
Jensen TS
影响因子：
30.8
作者：
Rustici, G;Mata, J;Bähler, J
通讯作者：
Bähler, J
影响因子：
64.5
作者：
Knott SR;Peace JM;Ostrow AZ;Gan Y;Rex AE;Viggiani CJ;Tavaré S;Aparicio OM
通讯作者：
Aparicio OM
影响因子：
3.3
作者：
Spellman, PT;Sherlock, G;Futcher, B
通讯作者：
Futcher, B