Decreased Epicardial CTRP3 mRNA Levels in Patients with Type 2 Diabetes Mellitus and Coronary Artery Disease Undergoing Elective Cardiac Surgery: A Possible Association with Coronary Atherosclerosis.
Decreased Epicardial CTRP3 mRNA Levels in Patients with Type 2 Diabetes Mellitus and Coronary Artery Disease Undergoing Elective Cardiac Surgery: A Possible Association with Coronary Atherosclerosis.
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2型糖尿病合并冠状动脉疾病择期心脏手术患者心外膜CTRP3信使核糖核酸水平降低：可能与冠状动脉粥样硬化相关
DOI：
10.3390/ijms23179988
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发表时间：
2022-09-01
影响因子：
5.6
通讯作者：
Haluzik, Martin
中科院分区：
文献类型：
--
作者：
Matloch, Zdenek;Mraz, Milos;Kasperova, Barbora Judita;Kratochvilova, Helena;Svoboda, Petr;Pleyerova, Iveta;Reznickova, Katerina;Norman, Sarah;Hlavacek, Daniel;Mahrik, Jakub;Ivak, Peter;Lacinova, Zdenka;Netuka, Ivan;Haluzik, Martin
关键词：
（1）背景：C1q TNF相关蛋白3（CTRP3）是一种具有抗炎和心脏保护特性的脂肪因子。在我们的研究中，我们探讨了接受择期心脏手术的患有或不患有冠状动脉疾病（CAD）和2型糖尿病（T2DM）的患者血清CTRP3及其在心外膜（EAT）和皮下（SAT）脂肪组织中基因表达的变化。 (2) 方法：在手术开始和结束时收集 34 名患者的 SAT、EAT 和血液样本：(i) 11 名无 CAD 或 T2DM，(ii) 14 名有 CAD 但无 T2DM，(iii) 9 名同时患有 CAD 和 T2DM。通过定量逆转录 PCR 评估 CTRP3 的 mRNA 水平。使用 ELISA 和 Luminex Multiplex 商业试剂盒测量 CTRP3 和其他因子的循环水平。 (3)结果：TNF-α和IL6的基线血浆水平在各组之间没有差异，并且在手术结束时升高。 CTRP3 的基线循环水平在各组之间没有差异，并且在手术后下降。相比之下，CAD/T2DM组中EAT的基线CTRP3 mRNA水平显着降低，而TNF-α和IL6基因表达没有发现差异。 (4) 结论：我们的数据表明，CTRP3 的 EAT mRNA 水平降低可能导致 CAD 和 T2DM 患者动脉粥样硬化的风险增加。
(1) Background: C1q TNF-related protein 3 (CTRP3) is an adipokine with anti-inflammatory and cardioprotective properties. In our study, we explored changes in serum CTRP3 and its gene expression in epicardial (EAT) and subcutaneous (SAT) adipose tissue in patients with and without coronary artery disease (CAD) and type 2 diabetes mellitus (T2DM) undergoing elective cardiac surgery. (2) Methods: SAT, EAT, and blood samples were collected at the start and end of surgery from 34 patients: (i) 11 without CAD or T2DM, (ii) 14 with CAD and without T2DM, and (iii) 9 with both CAD and T2DM. mRNA levels of CTRP3 were assessed by quantitative reverse transcription PCR. Circulating levels of CTRP3 and other factors were measured using ELISA and Luminex Multiplex commercial kits. (3) Results: Baseline plasma levels of TNF-α and IL6 did not differ among the groups and increased at the end of surgery. Baseline circulating levels of CTRP3 did not differ among the groups and decreased after surgery. In contrast, baseline CTRP3 mRNA levels in EAT were significantly decreased in CAD/T2DM group, while no differences were found for TNF-α and IL6 gene expression. (4) Conclusions: Our data suggest that decreased EAT mRNA levels of CTRP3 could contribute to higher risk of atherosclerosis in patients with CAD and T2DM.
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影响因子：
4.6
作者：
Koch, Alexander;Weiskirchen, Ralf;Tacke, Frank
通讯作者：
Tacke, Frank
影响因子：
3.7
作者：
Corradi, D;Maestri, R;Bordi, C
通讯作者：
Bordi, C
影响因子：
4.8
作者：
Deng W;Li C;Zhang Y;Zhao J;Yang M;Tian M;Li L;Zheng Y;Chen B;Yang G
通讯作者：
Yang G
来自成熟树突状细胞的外泌体通过膜 TNF-α 介导的 NF-κ B 途径增加内皮炎症和动脉粥样硬化
影响因子：
5.3
作者：
Gao W;Liu H;Yuan J;Wu C;Huang D;Ma Y;Zhu J;Ma L;Guo J;Shi H;Zou Y;Ge J
通讯作者：
Ge J
DOI：
10.1530/joe-16-0211
发表时间：
2016-12
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Luo L;Liu M
通讯作者：
Liu M