Rational Fabrication of Folate-Conjugated Zein/Soy Lecithin/Carboxymethyl Chitosan Core-Shell Nanoparticles for Delivery of Docetaxel.
Rational Fabrication of Folate-Conjugated Zein/Soy Lecithin/Carboxymethyl Chitosan Core-Shell Nanoparticles for Delivery of Docetaxel.
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DOI：
10.1021/acsomega.2c01270
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发表时间：
2022-04-19
期刊：
影响因子：
4.1
通讯作者：
Liu, Guijin
中科院分区：
文献类型：
--
作者：
Wu, Zhenyao;Li, Jie;Zhang, Xin;Li, Yangjia;Wei, Dongwei;Tang, Lichang;Deng, Shiming;Liu, Guijin
这项工作的目的是设计和制造一种具有核壳结构的天然玉米醇溶蛋白基纳米复合材料，用于输送抗癌药物。在设计方面，合成了叶酸缀合的玉米醇溶蛋白（Fa-zein）作为内部疏水核心；选择大豆卵磷脂（SL）和羧甲基壳聚糖（CMC）作为形成外壳的涂层成分。在制造方面，建立了一种新颖且合适的雾化/反溶剂沉淀工艺。结果表明，在合适的Fa-zein/SL/CMC质量比（100:30:10）下成功制备了Fa-zein/SL/CMC核壳纳米粒子（FZLC NPs），冻干FZLC粉末在水中表现出良好的再分散性和稳定性。之后，将多西他赛（DTX）作为模型药物以不同的DTX与FZLC（MR）质量比封装到FZLC NPs中。当MR = 1:15时，获得的DTX/FZLC纳米粒子具有高包封率（79.22±0.37%）、小粒径（206.9±48.73 nm）和高zeta电位（−41.8±3.97 mV）。 DTX以非晶态分散在FZLC基质的内核中。结果证明，DTX/FZLC NPs可以增加DTX的溶出，维持DTX的释放，并显着增强DTX的细胞毒性。本研究提供了对用于递送抗癌药物的玉米醇溶蛋白基复合纳米载体的形成的见解。
The objective of this work is to design and fabricate a natural zein-based nanocomposite with core–shell structure for the delivery of anticancer drugs. As for the design, folate-conjugated zein (Fa-zein) was synthesized as the inner hydrophobic core; soy lecithin (SL) and carboxymethyl chitosan (CMC) were selected as coating components to form an outer shell. As for fabrication, a novel and appropriate atomizing/antisolvent precipitation process was established. The results indicated that Fa-zein/SL/CMC core–shell nanoparticles (FZLC NPs) were successfully produced at a suitable mass ratio of Fa-zein/SL/CMC (100:30:10) and the freeze-dried FZLC powder showed a perfect redispersibility and stability in water. After that, docetaxel (DTX) as a model drug was encapsulated into FZLC NPs at different mass ratios of DTX to FZLC (MR). When MR = 1:15, DTX/FZLC NPs were obtained with high encapsulation efficiency (79.22 ± 0.37%), small particle size (206.9 ± 48.73 nm), and high zeta potential (−41.8 ± 3.97 mV). DTX was dispersed in the inner core of the FZLC matrix in an amorphous state. The results proved that DTX/FZLC NPs could increase the DTX dissolution, sustain the DTX release, and enhance the DTX cytotoxicity significantly. The present study provides insight into the formation of zein-based complex nanocarriers for the delivery of anticancer drugs.
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DOI：
10.1016/j.msec.2021.112331
发表时间：
2021-07-24
影响因子：
7.9
作者：
Gagliardi, Agnese;Voci, Silvia;Cosco, Donato
通讯作者：
Cosco, Donato
影响因子：
5.8
作者：
Hong, Soon-Seok;Thapa, Raj Kumar;Lim, Soo-Jeong
通讯作者：
Lim, Soo-Jeong
影响因子：
4
作者：
Khosravian P;Shafiee Ardestani M;Khoobi M;Ostad SN;Dorkoosh FA;Akbari Javar H;Amanlou M
通讯作者：
Amanlou M
影响因子：
3.3
作者：
Karthikeyan, K.;Lakra, Rachita;Korrapati, Purna Sai
通讯作者：
Korrapati, Purna Sai
影响因子：
14.9
作者：
GERAGHTY, D;PEIFER, MA;MESSING, J
通讯作者：
MESSING, J