Bifidobacteria isolated from vaginal and gut microbiomes are indistinguishable by comparative genomics.
Bifidobacteria isolated from vaginal and gut microbiomes are indistinguishable by comparative genomics.
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DOI：
10.1371/journal.pone.0196290
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Hill JE
中科院分区：
文献类型：
--
作者：
Freitas AC;Hill JE
双歧杆菌在人的胃肠道、阴道、口腔和母乳中定植。它们通过促进健康影响人体生理和营养，作为新生儿肠道的主要定植菌发挥重要作用，并通过产生乳酸来促进阴道微生物组的稳态。然而，双歧杆菌从母亲传染给婴儿的机制仍在讨论中。此外，研究表明双歧杆菌具有肠道定植的专门化，但缺乏来自不同身体部位的同一双歧杆菌种类的菌株的比较。在这里，我们的目的是比较短双歧杆菌（n = 17）和长双歧杆菌（n = 26）的基因组，以评估两种细菌的肠道和阴道分离株是否可以根据基因组含量进行区分。对短芽孢杆菌和长芽孢杆菌的一般基因组特征比较表明，阴道和肠道分离株在大小、GC含量、基因数量和CRISPR方面没有差异。平均核苷酸鉴定和全基因组系统发育分析显示，阴道和肠道分离株没有单独聚类。阴道和肠道分离株也具有相似的COG（簇同源群）类别分布。阴道菌株和肠道菌株之间的辅助基因组存在差异，但不足以根据其来源区分分离株。本研究的结果支持了阴道和肠道微生物群由双歧杆菌共同群落定植的假设，并进一步强调了母体阴道微生物群作为婴儿肠道微生物群来源的潜在重要性。
Bifidobacteria colonize the human gastrointestinal tract, vagina, oral cavity and breast milk. They influence human physiology and nutrition through health-promoting effects, play an important role as primary colonizers of the newborn gut, and contribute to vaginal microbiome homeostasis by producing lactic acid. Nevertheless, the mechanisms by which bifidobacteria are transmitted from mother to infant remains in discussion. Moreover, studies have suggested that Bifidobacterium spp. have specializations for gut colonization, but comparisons of strains of the same bifidobacteria species from different body sites are lacking. Here, our objective was to compare the genomes of Bifidobacterium breve (n = 17) and Bifidobacterium longum (n = 26) to assess whether gut and vaginal isolates of either species were distinguishable based on genome content. Comparison of the general genome features showed that vaginal and gut isolates did not differ in size, GC content, number of genes and CRISPR, either for B. breve or B. longum. Average nucleotide identity and whole genome phylogeny analysis revealed that vaginal and gut isolates did not cluster separately. Vaginal and gut isolates also had a similar COG (Cluster of Orthologous Group) category distribution. Differences in the accessory genomes between vaginal and gut strains were observed, but were not sufficient to distinguish isolates based on their origin. The results of this study support the hypothesis that the vaginal and gut microbiomes are colonized by a shared community of Bifidobacterium, and further emphasize the potential importance of the maternal vaginal microbiome as a source of infant gut microbiota.
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影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
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DOI：
10.1016/j.ijfoodmicro.2010.10.036
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Gueimonde, Miguel
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Jun, Wang
影响因子：
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Ventura M