The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors.
The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors.
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DOI：
10.1093/plcell/koad036
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发表时间：
2023-04-20
期刊：
影响因子：
11.6
通讯作者：
Talbot, Nicholas J.
中科院分区：
文献类型：
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作者：
Yan, Xia;Tang, Bozeng;Ryder, Lauren S.;MacLean, Dan;Were, Vincent M.;Eseola, Alice Bisola;Cruz-Mireles, Neftaly;Ma, Weibin;Foster, Andrew J.;Oses-Ruiz, Miriam;Talbot, Nicholas J.
稻瘟病菌是一种严重威胁全球水稻生产的毁灭性病害。尽管进行了大量的研究，但对稻瘟病期间植物组织入侵的生物学仍然知之甚少。在这里，我们报告了一个高分辨率的转录谱研究整个植物相关的发展的稻瘟病菌。我们的分析揭示了植物感染过程中真菌基因表达的主要时间变化。病原体基因表达可分为10个模块的时间共表达基因，提供证据的诱导显着的转变，在初级和次级代谢，细胞信号传导和转录调控。一组863个编码分泌蛋白的基因在感染的特定阶段差异表达，并且预测546个命名为MEP（Magnaportheeffector protein）的基因编码效应子。计算预测的结构相关的MEP，包括MAX效应器家族，揭示了他们的时间在相同的共表达模块的共同调节。我们表征了32个MEP基因，并证明Mep效应子主要通过活体营养界面复合物靶向水稻细胞的细胞质，并使用共同的非常规分泌途径。综上所述，我们的研究揭示了与爆震病相关的基因表达的主要变化，并确定了成功感染的关键效应子的多样性。转录谱揭示了广泛的效应库是如何部署在水稻稻瘟病，包括时间共调节，序列无关，结构保守的效应。
The rice blast fungus Magnaporthe oryzae causes a devastating disease that threatens global rice (Oryza sativa) production. Despite intense study, the biology of plant tissue invasion during blast disease remains poorly understood. Here we report a high-resolution transcriptional profiling study of the entire plant-associated development of the blast fungus. Our analysis revealed major temporal changes in fungal gene expression during plant infection. Pathogen gene expression could be classified into 10 modules of temporally co-expressed genes, providing evidence for the induction of pronounced shifts in primary and secondary metabolism, cell signaling, and transcriptional regulation. A set of 863 genes encoding secreted proteins are differentially expressed at specific stages of infection, and 546 genes named MEP (Magnaportheeffector protein) genes were predicted to encode effectors. Computational prediction of structurally related MEPs, including the MAX effector family, revealed their temporal co-regulation in the same co-expression modules. We characterized 32 MEP genes and demonstrate that Mep effectors are predominantly targeted to the cytoplasm of rice cells via the biotrophic interfacial complex and use a common unconventional secretory pathway. Taken together, our study reveals major changes in gene expression associated with blast disease and identifies a diverse repertoire of effectors critical for successful infection. Transcriptional profiling reveals how an extensive effector repertoire is deployed during rice blast disease, including temporally co-regulated, sequence-unrelated, structurally conserved effectors.
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